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重组人白血病抑制因子(LIF)

Human LIF Protein

产品货号
PRP1232

产品特点:

  • 高生物活性:经TF-1细胞增殖实验验证,ED50 = 1.079 ng/mL
  • 极低内毒素:<0.1 EU/μg (LAL法测定)
  • 严格质控:>98%纯度(SDS-PAGE验证)
  • 明确分子量:22.1 kDa精准匹配
  • 无载体干扰:未添加动物源载体蛋白
  • 选择规格

    5 μg
    ¥588
    现货(次日发货)
    20 μg
    ¥1498
    现货(次日发货)
    100 μg
    ¥3216
    现货(次日发货)
    1 mg
    ¥请咨询
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    活动截止时间:2024年1月31日

    产品概述

    重组人白血病抑制因子(LIF)蛋白(货号:PRP1232)是由大肠杆菌表达系统生产的无标签重组蛋白,其氨基酸序列来源于人源LIF (Ser23-Phe202) (P15018-1)。该产品以冻干粉形式提供(冻干缓冲液:无菌20 mM Tris,50 mM NaCl,pH 8.0),分子量为22.1 kDa,经SDS-PAGE验证纯度>98%,适用于细胞培养等研究领域。

    白血病抑制因子(Leukemia Inhibitory Factor, LIF)属于IL-6细胞因子超家族,广泛表达于多种组织和细胞类型。LIF通过异二聚体受体复合物(LIF-R/gp130)激活下游Erk、PI3K和Jak1/Stat1/3信号通路,具有多效性生物学功能:维持小鼠胚胎干细胞多能性与自我更新的关键因子;在肌肉组织中诱导成肌细胞增殖,参与运动诱导的肌肉肥大;同时可负向调节Th2和Th17细胞分化,在免疫调节中发挥重要作用。

    应用领域

    • 干细胞研究:维持胚胎干细胞多能性与自我更新
    • 肌肉生物学:成肌细胞增殖与分化机制研究
    • 免疫调节:Th细胞亚群分化调控实验
    • 信号通路研究:IL-6超家族细胞因子功能解析
    • 疾病模型构建:炎症性疾病与肿瘤微环境研究

    保存与使用提示:冻干粉需-20℃以下干燥保存,复溶推荐使用配套复溶液(浓度≥100 µg/mL),复溶后4℃可保存2-7天,长期保存需添加0.1% HSA/BSA并分装存于-20℃以下,避免反复冻融。使用前请离心处理冻干粉。

    重组人白血病抑制因子(LIF)

    产品参数

    中文名称 重组人白血病抑制因子(LIF)
    英文名称 Human LIF Protein
    产品货号 PRP1232
    序列 氨基酸序列来源于:人源 LIF (Ser23-Phe202) (P15018-1)表达的蛋白片段。
    标签 无标签
    种属
    活性 通过其在TF-1细胞中诱导增殖的能力来测定。该效应的ED50为1.079 ng/mL。
    蛋白长度 重组人LIF 由180个氨基酸组成,预测分子量为22.1 KD。
    表达宿主 大肠杆菌
    纯度 > 98 % ,使用SDS-PAGE检测
    产品形式 冻干粉,冻干缓冲液为无菌的20 mM Tris,50 mM NaCl,pH 8.0。
    分子量 22.1 kDa
    基因ID 2668
    蛋白质ID P15018-1
    蛋白序列链接 LIF - Leukemia inhibitory factor - Homo sapiens (Human) | UniProtKB | UniProt
    分子 LIF
    检测指标 LIF
    注意事项 开盖使用前,请先离心。本产品为冻干粉,推荐用该产品配套的Reconstitution Buffer进行复溶,且浓度不低于100 µg/mL。客户可根据后续的实验需求,进行进一步的稀释。
    保存建议 本产品为冻干粉形式,建议:将冻干粉产品保存于-20℃以下的干燥环境中。本产品溶解之后,可在4℃保存2-7天,长期使用请置于-20℃以下。为长期保存,推荐添加载体蛋白(0.1% HSA或者BSA),并酌情分装保存,避免反复冻融。
    运输条件 冰袋运输(蓝冰)
    警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

    使用说明

    Technical Information

    • Sequence: Amino acid sequence derived from Human LIF (Ser23-Phe202) (P15018-1) was expressed.
    • Protein length: The recombinant Human LIF Protein consists of 180 amino acids and predicts a molecular mass of 22.1 kDa.
    • Biological Activity: Measured by its ability to induce proliferation in TF-1 cells. The ED50 for this effect is 1.079 ng/mL.
    • Endotoxin: <0.1 EU per μg of the protein by the LAL method.
    • Formulation: Lyophilized from sterile 20 mM Tris, 50 mM NaCl, pH8.0.
    • Sequence: Amino acid sequence derived from Human LIF (Ser23-Phe202) (P15018-1) was expressed.
    • Protein length: The recombinant Human LIF Protein consists of 180 amino acids and predicts a molecular mass of 22.1 kDa.
    • Biological Activity: Measured by its ability to induce proliferation in TF-1 cells. The ED50 for this effect is 1.079 ng/mL.
    • Endotoxin: <0.1 EU per μg of the protein by the LAL method.
    • Formulation: Lyophilized from sterile 20 mM Tris, 50 mM NaCl, pH8.0.
  • Sequence: Amino acid sequence derived from Human LIF (Ser23-Phe202) (P15018-1) was expressed.
  • Sequence:
  • Protein length: The recombinant Human LIF Protein consists of 180 amino acids and predicts a molecular mass of 22.1 kDa.
  • Protein length:
  • Biological Activity: Measured by its ability to induce proliferation in TF-1 cells. The ED50 for this effect is 1.079 ng/mL.
  • Biological Activity:
  • Endotoxin: <0.1 EU per μg of the protein by the LAL method.
  • Endotoxin:
  • Formulation: Lyophilized from sterile 20 mM Tris, 50 mM NaCl, pH8.0.
  • Formulation:

    Storage Instructions

    Lyophilized protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

    Lyophilized protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

    Usage Notes

    Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

    Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

    Figure

    SDS-PAGE analysis of Human LIF Protein.

    SDS-PAGE analysis of Human LIF Protein.

    Note: The product listed herein is for research use only and is not intended for use in Mouse or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

    Note: The product listed herein is for research use only and is not intended for use in Mouse or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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