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重组小鼠白介素9,His-标签(无动物源)(IL-9)

Mouse IL-9 Protein, His tag (Animal-Free)

产品货号
PRP1112

产品特点:

  • 超高生物活性:比活性>5×10⁶ IU/mg,ED₅₀<0.2 ng/mL
  • 极低内毒素:<0.1 EU/μg(LAL法检测)
  • 严格质控:SDS-PAGE纯度>98%
  • 无动物源体系:全程避免动物源性成分
  • 稳定性保障:冻干粉形态配合优化缓冲体系
  • 选择规格

    5 μg
    ¥588
    现货(次日发货)
    20 μg
    ¥1498
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    100 μg
    ¥4469
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    1 mg
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    活动截止时间:2024年1月31日

    产品概述

    亚科因重组小鼠白介素9(无动物源)蛋白(Mouse IL-9 Protein, His tag, Animal-Free)是通过大肠杆菌表达系统制备的高纯度细胞因子,包含小鼠IL-9的功能结构域(Gln9-Pro144),C端融合His标签,以冻干粉形式提供,适用于细胞培养及相关研究。

    白细胞介素9(IL-9)是由P15247基因编码的多效性细胞因子,属于白细胞介素家族信号分子。其主要由Th9细胞、肥大细胞及Treg细胞分泌,在免疫调节和炎症反应中起关键作用。本产品通过MO7e细胞增殖实验验证生物活性:ED50值<0.2 ng/mL,比活性>5×106 IU/mg。采用无动物源生产工艺,显著降低外源因子污染风险,为Th2细胞分化、哮喘机制和肿瘤免疫微环境研究提供可靠工具。

    应用领域

    本品专为细胞水平研究设计,主要应用于:T淋巴细胞亚群分化研究、肥大细胞功能调控实验、肿瘤免疫微环境模拟、自身免疫性疾病机制探索等细胞培养体系,尤其适用于需要避免外源干扰的精细化实验。

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    重组小鼠白介素9,His-标签(无动物源)(IL-9)

    产品参数

    中文名称 重组小鼠白介素9,His-标签(无动物源)(IL-9)
    英文名称 Mouse IL-9 Protein, His tag (Animal-Free)
    产品货号 PRP1112
    序列 氨基酸序列来源于:小鼠源IL-9 (Gln9-Pro144) (P15247)
    标签 His
    种属 小鼠
    活性 Measure by its ability to induce proliferation in MO7e cells. The ED₅₀ for this effect is <0.2 ng/mL. The specific activity of recombinant mouse IL-9 is > 5 x 10⁶ IU/mg.
    蛋白长度 重组人IL-9 蛋白由136个氨基酸组成,预测分子量为14.97 KD。
    表达宿主 大肠杆菌
    纯度 > 98 % ,使用SDS-PAGE检测
    产品形式 冻干粉,冻干缓冲液为无菌的PBS,pH 7.4。
    分子量 14.97 kDa
    基因ID 16198
    蛋白质ID P15247
    蛋白序列链接 https://www.uniprot.org/uniprotkb/P15247/entry
    分子 IL-9
    检测指标 IL-9
    注意事项 开盖使用前,请先离心。本产品为冻干粉,推荐用该产品配套的Reconstitution Buffer进行复溶,且浓度不低于100 µg/mL。客户可根据后续的实验需求,进行进一步的稀释。
    保存建议 冻干蛋白应在 -20°C 下保存 1 年。复溶后,在 2°C 至 8°C 下最多可保存 1 周。 在含有载体蛋白或稳定剂的缓冲液(如 0.1% BSA、10%FBS、5%HSA)中进一步稀释后,分装的蛋白质应在 -20°C 或 -80°C 下保存 3-15 个月。避免反复冻融循环。
    运输条件 冰袋运输(蓝冰)
    警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

    使用说明

    Sequence

    Amino acid sequence derived from Mouse IL-9 (Gln9-Pro144) (P15247) was expressed with 6×His tag at the N-terminus.

    Protein length

    The recombinant mouse IL-9 consists of 136 amino acids and predicts a molecular mass of 14.97 kDa.

    Protein length

    The recombinant mouse IL-9 consists of 136 amino acids and predicts a molecular mass of 14.97 kDa.

    Biological Activity

    Measured by its ability to induce proliferation in MO7e cells. The ED50 for this effect is <0.2 ng/mL.

    Biological Activity

    Measured by its ability to induce proliferation in MO7e cells. The ED50 for this effect is <0.2 ng/mL.

    Endotoxin

    <0.1 EU per 1 μg of the protein by the LAL method.

    Endotoxin

    <0.1 EU per 1 μg of the protein by the LAL method.

    Formulation

    Lyophilized from PBS, pH 7.4.

    Formulation

    Lyophilized from PBS, pH 7.4.

    Storage Instructions

    Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA, 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.

    Storage Instructions

    Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA, 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.

    Usage notes

    Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.

    Usage notes

    Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.

    Fig. SDS-PAGE analysis of Mouse IL-9 protein.

    Not for further distribution without written consent. Copyright © Abbkine, Inc.

    Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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