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重组人Dickkopf相关蛋白1(DKK-1)
Human DKK-1 Protein
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活动截止时间:2024年1月31日
重组人Dickkopf相关蛋白1(Human DKK-1 Protein)是一种在大肠杆菌中表达的高纯度重组蛋白,适用于细胞培养及相关研究领域。该蛋白以冻干粉形式提供,冻干缓冲液为无菌PBS(pH 7.4),便于复溶后用于细胞实验。
Human DKK-1是一种分泌型拮抗剂,靶向Wnt/β-Catenin信号通路。它通过与LRP-6受体相互作用,形成DKK-1/LRP-6/Kremen 2复合物并内化,从而有效下调Wnt信号传导。Wnt信号与DKK-1抑制之间的平衡对骨形成和稳态维持至关重要;此外,DKK-1在癌症研究中具有双重作用,可作为肿瘤抑制因子或转移促进因子。
本产品主要应用于细胞培养实验,聚焦于Wnt信号通路调控研究,包括骨代谢、癌症转移机制及细胞稳态等领域。
| 中文名称 | 重组人Dickkopf相关蛋白1(DKK-1) |
| 英文名称 | Human DKK-1 Protein |
| 产品货号 | PRP1079 |
| 序列 | 氨基酸序列来源于: 表达人DKK-1 (Thr32-His266) (O94907)。 |
| 种属 | 人 |
| 蛋白长度 | 重组人DKK-1由235个氨基酸组成,预测分子量为25.8 kDa。 |
| 表达宿主 | 大肠杆菌 |
| 纯度 | > 98 %,使用SDS-PAGE检测 |
| 产品形式 | 冻干粉,冻干缓冲液为无菌的PBS, pH 7.4. |
| 分子量 | 25.8 kDa |
| 基因ID | 22943 |
| 蛋白质ID | O94907 |
| 蛋白序列链接 | `https://www.uniprot.org/uniprotkb/O94907/entry |
| 分子 | DKK-1 |
| 检测指标 | DKK-1 |
| 注意事项 | 开盖使用前,请先离心。本产品为冻干粉,推荐用该产品配套的Reconstitution Buffer进行复溶,且浓度不低于100 µg/mL。客户可根据后续的实验需求,进行进一步的稀释。 |
| 保存建议 | 冻干蛋白应在 -20°C 下保存 1 年。复溶后,在 2°C 至 8°C 下最多可保存 1 周。 在含有载体蛋白或稳定剂的缓冲液中进一步稀释(如 0.1%HSA或BSA)中进一步稀释,蛋白等分应在 -20°C 或 -80°C 下保存 3-6 个月。避免反复冻融循环。 |
| 运输条件 | 冰袋运输(蓝冰) |
| 警告 | 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。 |
Amino acid sequence derived from Human DKK-1 (Thr32-His266) (O94907) was expressed.
The recombinant Human DKK-1 consists of 235 amino acids and predicts a molecular mass of 25.8 kDa.
Testing in Progress.
<1 EU per μg of the protein by the LAL method.
Lyophilized from sterile PBS, pH7.4.
Lyophilized protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Lyophilized protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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