分享产品
重组大鼠碱性成纤维细胞生长因子(bFGF)
Rat bFGF protein
产品特点:
选择规格
买2送1活动进行中!购买任意2个规格产品,免费赠送100μL装一支。
活动截止时间:2024年1月31日
买2送1活动进行中!购买任意2个规格产品,免费赠送100μL装一支。
活动截止时间:2024年1月31日
重组大鼠碱性成纤维细胞生长因子(bFGF)蛋白(货号:PRP1210)是一种通过大肠杆菌表达系统生产的重组细胞因子,专为细胞培养和研究设计。该蛋白基于大鼠源bFGF亚型(P13109)的特定氨基酸序列(Ala11-Ser154)表达,采用冻干粉形式提供,缓冲液为无菌的20 mM Tris,150 mM NaCl,pH 8.0,适用于体外细胞增殖、分化及相关生物实验。
碱性成纤维细胞生长因子(bFGF),也称为FGF2,是成纤维细胞生长因子(FGF)家族的关键成员,在调节正常生理过程中发挥核心作用,包括血管生成、伤口愈合、组织修复、胚胎发育分化以及神经元功能。其转基因过度表达可能导致过度增殖和血管生成等病理状态,因此,重组大鼠bFGF蛋白成为研究这些机制的重要工具。本产品通过Balb/c 3T3小鼠胚胎成纤维细胞增殖试验验证活性,ED50值为0.6 ng/mL,确保其在细胞培养应用中高效促进生长和分化。
本产品主要应用于细胞培养领域,作为核心细胞因子,广泛用于血管生成研究、伤口愈合机制探索、神经退行性疾病模型、胚胎发育分化实验以及组织工程研究,支持各类体外细胞增殖和功能分析。
| 中文名称 | 重组大鼠碱性成纤维细胞生长因子(bFGF) |
| 英文名称 | Rat bFGF protein |
| 产品货号 | PRP1210 |
| 序列 | 氨基酸序列来源于:鼠源 bFGF亚型(P13109)(Ala11-Ser154)表达的蛋白片段。 |
| 标签 | 无标签 |
| 种属 | 大鼠 |
| 活性 | 使用Balb/c 3T3小鼠胚胎成纤维细胞进行细胞增殖试验。这种效应的ED50为0.6 ng/mL。 |
| 蛋白长度 | 重组大鼠bFGF由145个氨基酸组成,预测分子量为16.2 KD。在还原性条件下的SDS-PAGE中,重组大鼠bFGF的分子量约为16.2 KD。 |
| 表达宿主 | 大肠杆菌 |
| 纯度 | > 95 % ,使用SDS-PAGE检测 |
| 产品形式 | 冻干粉,冻干缓冲液为无菌的20 mM Tris,150 mM NaCl,pH 8.0。 |
| 分子量 | 16.2 kDa |
| 基因ID | 54250 |
| 蛋白质ID | P13109 |
| 蛋白序列链接 | https://www.uniprot.org/uniprot/P13109 |
| 分子 | bFGF |
| 注意事项 | 开盖使用前,请先离心。本产品为冻干粉,推荐用该产品配套的Reconstitution Buffer进行复溶,且浓度不低于100 µg/mL。客户可根据后续的实验需求,进行进一步的稀释。 |
| 保存建议 | 本产品为冻干粉形式,建议:将冻干粉的重组大鼠碱性成纤维细胞生长因子(bFGF)产品保存于-20℃以下的干燥环境中。本产品溶解之后,可在4℃保存2-7天,长期使用请置于-20℃以下。为长期保存,推荐添加载体蛋白(0.1% HSA或者BSA),并酌情分装保存,避免反复冻融。 |
| 运输条件 | 冰袋运输(蓝冰) |
| 警告 | 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。 |
Amino acid sequence derived from Rat FGF2 isoform (P13109) (Ala11-Ser154) was expressed.
The recombinant consists of 145 amino acids (N-Met)and has a predicted molecular mass of 16.2 kDa.
<1.0 EU per μg of the protein as determined by the LAL method.
Lyophilized from sterile 20 mM Tris, 150 mM NaCl, pH 8.0.
Lyophilized Rat FGF2 protein protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Lyophilized Rat FGF2 protein protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Rat FGF2 protein protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Rat FGF2 protein protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Fig. SDS-PAGE analysis of Rat FGF2 protein.
Fig. SDS-PAGE analysis of Rat FGF2 protein.
Fig. SDS-PAGE analysis of Rat FGF2 protein.
Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.0015 ng/mL. The specific activity of recombinant Rat bFGF is approximately >6.67 x 108 IU/mg.
10-6 10-4 10-2 100 102
100
110
120
130
140
Rat bFGF (ng/mL)
Proliferation %
Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.0015 ng/mL. The specific activity of recombinant Rat bFGF is approximately >6.67 x 108 IU/mg.
810-6 10-4 10-2 100 102
100
110
120
130
140
Rat bFGF (ng/mL)
Proliferation %
10-6 10-4 10-2 100 102
100
110
120
130
140
Rat bFGF (ng/mL)
Proliferation %
10-6 10-4 10-2 100 102
-6-4-202100
110
120
130
140
Rat bFGF (ng/mL)
Proliferation %
Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
暂无相关常见问题。如有疑问,请联系我们的技术支持团队。
请等待最新文献信息更新。
我们的技术团队为您提供全方位的产品支持服务
根据您的研究目标,提供个性化的实验设计方案
经验丰富的技术专家协助您解决实验中遇到的问题
7x24小时在线技术支持,随时为您答疑解惑
提供免费样品试用,让您先试后买更放心