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重组大鼠干扰素-γ(IFN-γ)

Rat IFN-γ protein

产品货号
PRP1214

产品特点:

  • 高纯度:≥95%通过SDS-PAGE检测,确保实验可靠性
  • 低内毒素水平:采用LAL法检测,每1μg蛋白质中EU含量小于1.0
  • 重组表达:在大肠杆菌中生产,无额外标签,保持天然生物活性
  • 冻干粉形式:缓冲液为无菌150mM NaCl, 20mM Tris, pH 7.0,便于储存和复溶
  • 选择规格

    20 μg
    ¥588
    现货(次日发货)
    100 μg
    ¥1498
    现货(次日发货)
    500 μg
    ¥4698
    现货(次日发货)
    1 mg
    ¥请咨询
    请咨询
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    活动截止时间:2024年1月31日

    产品概述

    重组大鼠干扰素-γ(IFN-γ)蛋白(货号:PRP1214)是一种通过大肠杆菌表达系统生产的重组细胞因子,用于细胞培养等研究中的免疫调节和抗病毒功能分析,适用于血清、细胞或组织样本的体外实验。

    IFN-γ,也称为IFNG,属于II型干扰素家族的一种分泌蛋白,其氨基酸序列来源于大鼠IFN-γ(P01581)的Glu23-Cys156片段。IFN-γ主要由自然杀伤性T细胞(NK)产生,在免疫应答中扮演关键角色,包括激活巨噬细胞、抑制肿瘤细胞增殖和增强I型干扰素的抗病毒作用。其结构包含一个有六个α螺旋的核心和未折叠序列,对抵抗病毒、细胞内细菌感染以及肿瘤控制至关重要。IFN-γ的异常表达与多种自身炎症和自身免疫疾病相关,凸显了其在免疫系统中的重要性。

    本产品主要应用于细胞培养研究,特别是在免疫学、病毒学和肿瘤学领域,用于探索IFN-γ的免疫调节、抗病毒及抗肿瘤机制。

    重组大鼠干扰素-γ(IFN-γ)

    产品参数

    中文名称 重组大鼠干扰素-γ(IFN-γ)
    英文名称 Rat IFN-γ protein
    产品货号 PRP1214
    序列 氨基酸序列来源于: 大鼠 IFN-γ (P01581)(Glu23-Cys156) 表达的蛋白片段。
    标签 无标签
    种属 大鼠
    蛋白长度 重组大鼠IFN-γ由134个氨基酸组成,预测分子量为14.8 kDa。 在还原条件下,在SDS-PAGE中大鼠IFN-γ蛋白的表观分子量约为14.8 kDa。
    表达宿主 大肠杆菌
    纯度 ≥ 95 % ,使用SDS-PAGE检测
    产品形式 冻干粉,冻干缓冲液为无菌的 150mM NaCl 20mM Tris , pH 7.0溶液。
    分子量 14.8 kDa
    基因ID 25712
    蛋白质ID P01581
    蛋白序列链接 https://www.uniprot.org/uniprot/P01581
    分子 IFN-γ
    注意事项 开盖使用前,请先离心。本产品为冻干粉,推荐用该产品配套的Reconstitution Buffer进行复溶,且浓度不低于100 µg/mL。客户可根据后续的实验需求,进行进一步的稀释。
    保存建议 本产品为冻干粉形式,建议:将冻干粉的重组大鼠干扰素-γ(IFN-γ)产品保存于-20℃以下的干燥环境中。本产品溶解之后,可在4℃保存2-7天,长期使用请置于-20℃以下。为长期保存,推荐添加载体蛋白(0.1% HSA或者BSA),并酌情分装保存,避免反复冻融。
    运输条件 常温运输
    警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

    使用说明

    Sequence

    Amino acid sequence derived from Rat IFN-γ isoform (P01581)(Glu23-Cys156) was expressed.

    Amino acid sequence derived from Rat IFN-γ isoform (P01581)(Glu23-Cys156) was expressed.

    Protein length

    The Rat IFN-γ consists of 134 amino acids and has a predicted molecular mass of 14.8 kDa. The apparent molecular mass of the Rat IFN-γ protein is approximately 14.8 kDa in SDS-PAGE under reducing conditions.

    The Rat IFN-γ consists of 134 amino acids and has a predicted molecular mass of 14.8 kDa. The apparent molecular mass of the Rat IFN-γ protein is approximately 14.8 kDa in SDS-PAGE under reducing conditions.

    Biological Activity

    Testing in progress.

    Testing in progress.

    Endotoxin

    < 1 EU per μg of the protein as determined by the LAL method.

    < 1 EU per μg of the protein as determined by the LAL method.

    Formulation

    Lyophilized from sterile 20 mM Tris, 150 mM NaCl pH 7.0.

    Lyophilized from sterile 20 mM Tris, 150 mM NaCl pH 7.0.

    Storage Instructions

    Lyophilized Rat IFN-γ protein product should be stored desiccated below -18°C for 12 months. Upon reconstitution, the protein should be stored at 4°C between 2 -7 days and below -18°C for 3 months. For long term storage it is recommended to add a carrier protein (example 0.1% HSA or BSA), aliquot and store below -18°C. Please prevent freeze-thaw cycles.

    Lyophilized Rat IFN-γ protein product should be stored desiccated below -18°C for 12 months. Upon reconstitution, the protein should be stored at 4°C between 2 -7 days and below -18°C for 3 months. For long term storage it is recommended to add a carrier protein (example 0.1% HSA or BSA), aliquot and store below -18°C. Please prevent freeze-thaw cycles.

    Usage notes

    Always centrifuge tubes before opening. It is recommended to centrifuge at 12,000 rpm for 1 minute. Then reconstitute the lyophilized Rat IFN-γ protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    Always centrifuge tubes before opening. It is recommended to centrifuge at 12,000 rpm for 1 minute. Then reconstitute the lyophilized Rat IFN-γ protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    Fig. SDS-PAGE analysis of Rat IFN-γ protein.

    Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

    Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

    常见问题

    暂无相关常见问题。如有疑问,请联系我们的技术支持团队。

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