重组人TNF相关的凋亡诱导配体,His-标签(无动物源)(TRAIL)

Name: 重组人TNF相关的凋亡诱导配体,His-标签(无动物源)(TRAIL)
Brand: Abbkine
SKU: PRP1208
Price: 588 CNY
Availability: InStock

Human TRAIL Protein, His tag (Animal-Free)

产品货号

PRP1208

产品特点：

超高纯度：经SDS-PAGE检测纯度＞98%，内毒素含量<0.1 EU/μg

严格活性验证：通过L929细胞毒性测试明确标注ED_₅₀值（10.4-15.4 ng/mL）

无动物源保障：全程无动物组分生产，降低实验干扰风险

稳定性优化：冻干粉形态配合专用复溶方案，活性保存长达15个月

选择规格

5 μg

¥588

现货（次日发货）

20 μg

¥1498

现货（次日发货）

100 μg

¥4869

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1 mg

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🎉 限时优惠

买2送1活动进行中！购买任意2个规格产品，免费赠送100μL装一支。

活动截止时间：2024年1月31日

产品概述

亚科因生物研发的重组人TNF相关的凋亡诱导配体，His-标签（无动物源）（Human TRAIL Protein, His tag (Animal-Free)）是一种通过大肠杆菌表达系统制备的高纯度细胞因子蛋白。该产品采用无动物源生产工艺，由167个氨基酸组成（对应人源TRAIL蛋白Arg115-Gly281结构域），冻干形态便于运输保存，专用于细胞凋亡机制研究与体外细胞实验。

TNF相关凋亡诱导配体（TRAIL）作为肿瘤坏死因子（TNF）超家族成员，是一种在胸腺、结肠、肺等成人组织中广泛表达的II型跨膜蛋白。其通过特异性激活死亡受体DR4/DR5触发凋亡信号通路，对乳腺癌细胞（MCF7）和上皮样癌细胞（HeLa）等具有显著促凋亡作用。本产品经L929细胞毒性实验验证（ED_₅₀=10.4-15.4 ng/mL），保留了天然TRAIL的生物活性功能，为肿瘤治疗机制研究提供关键工具。

核心应用领域

本品主要应用于肿瘤细胞凋亡机制研究、抗癌药物筛选模型构建及死亡受体信号通路解析，特别适用于：

肿瘤细胞系（如MCF7、HeLa）的体外凋亡诱导实验
DR4/DR5受体功能验证与抗体药物开发
细胞培养体系中凋亡相关蛋白的调控研究

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产品参数

中文名称	重组人TNF相关的凋亡诱导配体,His-标签(无动物源)(TRAIL)
英文名称	Human TRAIL Protein, His tag (Animal-Free)
产品货号	PRP1208
序列	氨基酸序列来源于：人源TRAIL Protein (Arg115-Gly281) (P50591-1)
标签	His
种属	人
活性	Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED₅₀ for this effect is 10.4-15.4 ng/mL.
蛋白长度	重组人TRAIL 蛋白由167个氨基酸组成，预测分子量为20.33 KD。
表达宿主	大肠杆菌
纯度	> 98 % ，使用SDS-PAGE检测
产品形式	冻干粉，冻干缓冲液为无菌的PBS，pH 8.0。
分子量	20.33 kDa
基因ID	8743
蛋白质ID	P50591
蛋白序列链接	https://www.uniprot.org/uniprotkb/P50591/entry
分子	TRAIL
检测指标	TRAIL
注意事项	开盖使用前，请先离心。本产品为冻干粉，推荐用该产品配套的Reconstitution Buffer进行复溶，且浓度不低于100 µg/mL。客户可根据后续的实验需求，进行进一步的稀释。
保存建议	冻干蛋白应在 -20°C 下保存 1 年。复溶后，在 2°C 至 8°C 下最多可保存 1 周。在含有载体蛋白或稳定剂的缓冲液（如 0.1% BSA、10%FBS、5%HSA 或 5% 海藻糖溶液）中进一步稀释后，分装的蛋白质应在 -20°C 或 -80°C 下保存 3-15 个月。避免反复冻融循环。
运输条件	冰袋运输（蓝冰）
警告	本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。

使用说明

Sequence

Amino acid sequence derived from Human TRAIL Protein (Arg115-Gly281) (P50591-1) was expressed with 6×His tag at the C-terminus.

Protein length

The recombinant Human TRAIL Protein consists of 167 amino acids and predicts a molecular mass of 20.33 kDa.

Protein length

The recombinant Human TRAIL Protein consists of 167 amino acids and predicts a molecular mass of 20.33 kDa.

Biological Activity

Measured by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED50 for this effect is 10.4-15.4 ng/mL.

Biological Activity

Measured by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED50 for this effect is 10.4-15.4 ng/mL.

Endotoxin

<0.1 EU per 1 μg of the protein by the LAL method.

Endotoxin

<0.1 EU per 1 μg of the protein by the LAL method.

Formulation

Lyophilized from PBS, pH 8.0.

Formulation

Lyophilized from PBS, pH 8.0.

Storage Instructions

Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.

Storage Instructions

Usage notes

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.

Usage notes

Fig. SDS-PAGE analysis of Human TRAIL Protein.

Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.