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重组小鼠白血病抑制因子,His-标签(无动物源)(LIF)
Mouse LIF Protein, His tag (Animal-Free)
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活动截止时间:2024年1月31日
买2送1活动进行中!购买任意2个规格产品,免费赠送100μL装一支。
活动截止时间:2024年1月31日
亚科因生物提供的重组小鼠白血病抑制因子(Mouse LIF Protein, His tag, Animal-Free)是一种无动物源表达的高纯度细胞因子,通过大肠杆菌表达系统制备,携带N端6×His标签。该产品采用冻干形式保存,适用于干细胞维持、细胞分化调控等精细细胞实验研究。
白血病抑制因子(LIF)属于IL-6受体家族多效糖蛋白,在神经发育、胚胎干细胞自我更新及造血调控中发挥核心作用。本品表达小鼠LIF功能结构域(Ser114-Phe203),与人类LIF具有79%序列同源性。其生物学活性通过诱导M1细胞分泌IL-6的能力验证,可双向调控细胞命运——既能促进DA1细胞增殖和特定细胞存活,又可诱导细胞分化与程序性凋亡,是研究细胞多能性维持和谱系定向分化的关键工具蛋白。
• 胚胎干细胞与诱导多能干细胞(iPSC)的自我更新培养
• 神经干细胞分化与神经退行性疾病机制研究
• 造血干细胞扩增及白血病细胞分化诱导实验
• 骨代谢研究中成骨细胞/破骨细胞平衡调控
• 生殖生物学中胚泡着床相关信号通路解析
| 中文名称 | 重组小鼠白血病抑制因子,His-标签(无动物源)(LIF) |
| 英文名称 | Mouse LIF Protein, His tag (Animal-Free) |
| 产品货号 | PRP1160 |
| 序列 | 氨基酸序列来源于:表达小鼠LIF的氨基酸序列(Ser114-Phe 203) (P01137),其N端带有6×His标签。 |
| 种属 | 小鼠 |
| 活性 | 以其诱导 M1 细胞分泌 IL-6 的能力来衡量。这种效应的 ED₅₀ <0.5 ng/mL。重组小鼠 LIF 的特异性活性大于 2 x 10⁶ IU/mg。 |
| 蛋白长度 | 该蛋白质的计算分子量为 20.68 kDa。在还原条件下,蛋白质迁移为 17-25 kDa(SDS-PAGE 分析)。 |
| 表达宿主 | 大肠杆菌 |
| 纯度 | > 95 % ,使用SDS-PAGE检测 |
| 产品形式 | 该蛋白质是由 0.2 µm 过滤溶液冻干而成,含 1X PBS,pH 7.4。如有任何疑问或特殊要求,请与我们联系。 |
| 分子量 | 20.68 kDa |
| 蛋白质ID | P09056 |
| 蛋白序列链接 | https://www.uniprot.org/uniprot/P22003 |
| 分子 | LIF |
| 检测指标 | LIF |
| 注意事项 | 开盖使用前,请先离心。本产品为冻干粉,推荐用该产品配套的Reconstitution Buffer进行复溶,且浓度不低于100 µg/mL。客户可根据后续的实验需求,进行进一步的稀释。 |
| 保存建议 | 冻干蛋白应在 -20°C 下保存 1 年。重组后,在 2°C 至 8°C 下可保存 1 周。 在含有载体蛋白或稳定剂的缓冲液(如 0.1% BSA、10%FBS、5%HSA)中进一步稀释后,蛋白等分应在 -20°C 或 -80°C 下保存 3-6 个月。避免反复冻融循环。 |
| 运输条件 | 冰袋运输(蓝冰) |
| 警告 | 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。 |
Amino acid sequence derived from Mouse LIF (Ser114-Phe 203) (P01137) was expressed with 6×His tag at the N-terminus.
The recombinant Mouse LIF Protein consists of 90 amino acids and predicts a molecular mass of 20.68 kDa.
The recombinant Mouse LIF Protein consists of 90 amino acids and predicts a molecular mass of 20.68 kDa.
Measured by its ability to induce IL-6 secretion in M1 cells. The ED50 for this effect is <0.5 ng/mL.
Measured by its ability to induce IL-6 secretion in M1 cells. The ED50 for this effect is <0.5 ng/mL.
<0.1 EU per μg of the protein as determined by the LAL method.
<0.1 EU per μg of the protein as determined by the LAL method.
Lyophilized from sterile PBS, pH 7.4.
Lyophilized from sterile PBS, pH 7.4.
Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.
Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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