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重组人Noggin蛋白,His-标签(Noggin)
Human Noggin Protein, His tag
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活动截止时间:2024年1月31日
                            
                                
                                买2送1活动进行中!购买任意2个规格产品,免费赠送100μL装一支。
活动截止时间:2024年1月31日
PRP1942重组人Noggin蛋白,His标签(Human Noggin Protein, His tag)是一种由大肠杆菌表达系统生产的重组蛋白,通过高效纯化工艺获得,适用于细胞培养及相关研究。该蛋白基于人源Noggin (Q13253)的氨基酸序列(Gln28-Cys232)表达,分子量为23.2 KDa,纯度>98%(经SDS-PAGE验证),产品形式为冻干粉,冻干缓冲液为无菌的20 mM Tris,50 mM NaCl,pH 8.0,确保实验的一致性和可靠性。
Noggin是一种分泌性多肽,由Noggin基因(基因ID:25317)编码,能特异性结合并灭活转化生长因子β(TGF-β)超家族信号蛋白,如骨形态发生蛋白-4(BMP4)。它在发育过程中起关键作用,通过高效扩散形成形态发生梯度,参与神经管融合、关节形成等过程。人类Noggin的氨基酸序列高度保守,与爪蟾、大鼠和小鼠同源。研究表明,Noggin突变与近端关节融合综合征(SYM1)和多重关节融合综合征(SYNS1)相关,这些疾病以多关节融合为主要特征。本产品为研究TGF-β信号通路、发育生物学及疾病机制提供了重要工具。
本产品广泛应用于细胞培养、干细胞分化研究、发育生物学实验以及TGF-β信号通路分析等领域,特别适用于细胞因子相关研究。
                                    | 中文名称 | 重组人Noggin蛋白,His-标签(Noggin) | 
| 英文名称 | Human Noggin Protein, His tag | 
| 产品货号 | PRP1942 | 
| 序列 | 氨基酸序列来源于:人源Noggin (Q13253) (Gln28-Cys232) 表达的蛋白片段。 | 
| 标签 | His | 
| 种属 | 人 | 
| 活性 | Testing in Progress | 
| 蛋白长度 | 重组人Noggin由206个氨基酸组成,预测分子量为23.2 KD。 | 
| 表达宿主 | 大肠杆菌 | 
| 纯度 | > 98 % ,使用SDS-PAGE检测 | 
| 产品形式 | 冻干粉,冻干缓冲液为无菌的20 mM Tris,50 mM NaCl,pH 8.0。 | 
| 分子量 | 23.2 KDa | 
| 基因ID | 25317 | 
| 蛋白质ID | Q13253 | 
| 蛋白序列链接 | NOG - Noggin - Homo sapiens (Human) | UniProtKB | UniProt | 
| 分子 | Noggin | 
| 检测指标 | Noggin | 
| 注意事项 | 开盖使用前,请先离心。本产品为冻干粉,推荐用该产品配套的Reconstitution Buffer进行复溶,且浓度不低于102 µg/mL。客户可根据后续的实验需求,进行进一步的稀释。 | 
| 保存建议 | 本产品为冻干粉形式,建议:将冻干粉产品保存于-20℃以下的干燥环境中。本产品溶解之后,可在4℃保存2-7天,长期使用请置于-20℃以下。为长期保存,推荐添加载体蛋白(0.1% HSA或者BSA),并酌情分装保存,避免反复冻融。 | 
| 运输条件 | 冰袋运输(蓝冰) | 
| 警告 | 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。 | 
Amino acid sequence derived from Human Noggin (Q13253) (Gln28-Cys232) was expressed with 6×His tag.
The recombinant Human Noggin Protein consists of 206 amino acids and predicts a molecular mass of 23.2 kDa.
The recombinant Human Noggin Protein consists of 206 amino acids and predicts a molecular mass of 23.2 kDa.
Testing in Progress.
Testing in Progress.
<0.1 EU per μg of the protein by the LAL method.
<0.1 EU per μg of the protein by the LAL method.
Lyophilized from sterile 20 mM Tris, 50 mM NaCl, pH8.0.
Lyophilized from sterile 20 mM Tris, 50 mM NaCl, pH8.0.
Lyophilized protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Lyophilized protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Note: The product listed herein is for research use only and is not intended for use in Human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Note: The product listed herein is for research use only and is not intended for use in Human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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