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亚科因生产的重组小鼠表皮生长因子（EGF）蛋白（货号PRP1957）是一种通过大肠杆菌表达系统制备的无标签细胞因子，适用于细胞培养及相关研究。该产品以冻干粉形式提供，纯度>95%，可高效促进特定细胞类型的增殖与分化。
表皮生长因子(EGF)是一种具有重要生物学功能的多肽，在体内外实验中能有效刺激表皮组织、上皮细胞及部分成纤维细胞的生长增殖。研究表明，EGF通过激活表皮生长因子受体(EGFR)，可调节肾脏远曲小管的镁离子重吸收过程，并参与TRPM6镁离子通道的调控。此外，该因子在神经研究中表现出诱导神经突生长的能力，如在椎实螺运动神经元的体外实验中能促进神经突触延伸。
• 小鼠细胞培养增殖与分化研究
• 上皮组织再生机制实验
• 肾脏离子通道调控模型构建
• 神经突生长体外诱导实验
| 中文名称 | 重组小鼠表皮生长因子(EGF) |
| 英文名称 | Mouse EGF protein |
| 产品货号 | PRP1957 |
| 序列 | Amino acid sequence derived from Mouse EGF (Met978-Arg 1029)(P01132) was expressed . |
| 标签 | 无标签 |
| 种属 | 小鼠 |
| 活性 | Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.017 ng/mL. The specific activity of recombinant Mouse EGF is approximately >5.89 x 107IU/mg |
| 蛋白长度 | 6.2 KDa |
| 表达宿主 | 大肠杆菌 |
| 纯度 | > 95% |
| 产品形式 | 冻干粉，冻干缓冲液为无菌的150 mM NaCl 20 mM Tris , pH 8.0。 |
| 基因ID | 13645 |
| 蛋白序列链接 | http://www.uniprot.org/uniprot/P01132 |
| 分子 | MEGF |
| 注意事项 | 开盖使用前，请先离心。本产品为冻干粉，推荐用该产品配套的Reconstitution Buffer进行复溶，且浓度不低于100 µg/mL。客户可根据后续的实验需求，进行进一步的稀释。 |
| 保存建议 | 冻干蛋白应在 -20°C 下保存 1 年。复溶后，在 2°C 至 8°C 下最多可保存 1 周。 在含有载体蛋白或稳定剂的缓冲液（如 0.1% BSA、10%FBS、5%HSA）中进一步稀释后，分装的蛋白质应在 -20°C 或 -80°C 下保存 3-6 个月。避免反复冻融循环。 |
| 运输条件 | 冰袋运输（蓝冰） |
| 警告 | 本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。 |
Amino acid sequence derived from Mouse EGF (Met978-Arg 1029)(P01132) was expressed.
The recombinant mouse EGF consists of 55 amino acids and predicts a molecular mass of 6.2 KDa.
The recombinant mouse EGF consists of 55 amino acids and predicts a molecular mass of 6.2 KDa.
<0.1 EU per 1 μg of the protein by the LAL method.
<0.1 EU per 1 μg of the protein by the LAL method.
Lyophilized from sterile 150 mM NaCl 20 mM Tris, pH 8.0.
Lyophilized from sterile 150 mM NaCl 20 mM Tris, pH 8.0.
Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA, 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA, 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Mouse EGF protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Mouse EGF protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously
Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.017 ng/mL. The specific activity of recombinant Mouse EGF is approximately >5.89 x 107 IU/mg.
Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.017 ng/mL. The specific activity of recombinant Mouse EGF is approximately >5.89 x 107 IU/mg.
| Mouse EGF (ng/mL) | Proliferation (%) |
|---|---|
| 10-6 | 100 |
| 10-4 | 150 |
| 10-2 | 200 |
| 100 | 250 |
| 102 | 250 |
| 104 | 250 |
| Mouse EGF (ng/mL) | Proliferation (%) |
|---|---|
| 10-6 | 100 |
| 10-4 | 150 |
| 10-2 | 200 |
| 100 | 250 |
| 102 | 250 |
| 104 | 250 |
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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