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重组小鼠粒细胞集落刺激因子(G-CSF)

Mouse G-CSF protein

产品货号
PRP1308

产品特点:

  • 高纯度:>99%纯度,通过SDS-PAGE严格检测
  • 低内毒素:每微克蛋白内毒素含量<1.0 EU,符合LAL法标准
  • 强生物活性:ED50为2.038 pg/mL,高效诱导M-NFS-60细胞增殖
  • 无标签设计:避免实验干扰,确保结果准确性
  • 稳定表达:源自大肠杆菌宿主,预测分子量为18.95 KD
  • 选择规格

    5 μg
    ¥588
    现货(次日发货)
    20 μg
    ¥1588
    现货(次日发货)
    100 μg
    ¥4398
    现货(次日发货)
    1 mg
    ¥请咨询
    请咨询
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    活动截止时间:2024年1月31日

    产品概述

    重组小鼠粒细胞集落刺激因子(G-CSF)蛋白(货号:PRP1308)是一种基于大肠杆菌表达系统生产的无标签高纯度细胞因子蛋白,专为细胞培养及生物医学研究设计,可高效支持细胞增殖与分化实验。

    粒细胞集落刺激因子(G-CSF)是一种关键的生长因子和细胞因子,属于激素样糖蛋白的CSF家族,由免疫细胞和内皮细胞等产生。它通过结合G-CSF受体激活多种信号通路,包括Akt和Jak2-STAT3通路,从而促进造血干细胞及祖细胞的存活、增殖、分化与动员。在临床前研究中,G-CSF被证实能改善心肌梗死后的心脏功能与灌注,通过血管生成机制和心肌细胞保护作用减少左心室重塑。此外,重组G-CSF已在临床血液学和肿瘤学中广泛应用,支持骨髓移植或治疗化疗相关的中性粒细胞减少症。

    本产品广泛应用于细胞培养、造血干细胞研究、心肌梗死模型探索、药物筛选及临床前实验等领域,特别适用于细胞因子相关机制研究。

    重组小鼠粒细胞集落刺激因子(G-CSF)

    产品参数

    中文名称 重组小鼠粒细胞集落刺激因子(G-CSF)
    英文名称 Mouse G-CSF protein
    产品货号 PRP1308
    序列 氨基酸序列来源于:重组小鼠粒细胞集落刺激因子(G-CSF) (P09920) (V31-A208) 表达的蛋白片段。
    标签 无标签
    种属 小鼠
    活性 通过其诱导M-NFS-60细胞增殖的能力来测定。ED50为2.038 pg/mL。
    蛋白长度 重组小鼠粒细胞集落刺激因子(G-CSF) 由178个氨基酸组成,预测分子量为18.95 KD。
    表达宿主 大肠杆菌
    纯度 > 99% ,使用SDS-PAGE检测
    产品形式 冻干粉,冻干缓冲液为无菌的20 mM Tris,150 mM NaCl,pH 7.4。
    分子量 18.95 KD
    基因ID 12985
    蛋白质ID P09920
    蛋白序列链接 https://www.uniprot.org/uniprotkb/P09920/entry
    分子 G-CSF; Colony Stimulating Factor 3; Granulocyte; GCSF
    检测指标 G-CSF; Colony Stimulating Factor 3; Granulocyte; GCSF
    注意事项 开盖使用前,请先离心。本产品为冻干粉,推荐用该产品配套的Reconstitution Buffer进行复溶,且浓度不低于100 µg/mL。客户可根据后续的实验需求,进行进一步的稀释。
    保存建议 本产品为冻干粉形式,建议:将冻干粉产品保存于-20℃以下的干燥环境中。本产品溶解之后,可在4℃保存2-7天,长期使用请置于-20℃以下。为长期保存,推荐添加载体蛋白(0.1% HAS或BSA),并酌情分装保存,避免反复冻融。
    运输条件 冰袋运输(蓝冰)
    警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

    使用说明

    Sequence

    Amino acid sequence derived from Mouse G-CSF protein (P 09920) (V 31-A 208) was expressed.

    Protein length

    The Recombinant Mouse G-CSF protein consists of 178 amino acids and predicts a molecular mass of 18.95 KDa

    Protein length

    The Recombinant Mouse G-CSF protein consists of 178 amino acids and predicts a molecular mass of 18.95 KDa

    Biological Activity

    Measured by its ability to induce proliferation in M-NFS-60 cells. The ED50 for this effect is 2.038 pg/mL.

    Biological Activity

    Measured by its ability to induce proliferation in M-NFS-60 cells. The ED50 for this effect is 2.038 pg/mL.

    Endotoxin

    < 1.0 EU per μg of the protein as determined by the LAL method.

    Endotoxin

    < 1.0 EU per μg of the protein as determined by the LAL method.

    Formulation

    Lyophilized from sterile 20 mM Tris with 150 mM NaCl, pH 7.4.

    Formulation

    Lyophilized from sterile 20 mM Tris with 150 mM NaCl, pH 7.4.

    Storage Instructions

    Lyophilized Mouse G-CSF protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

    Storage Instructions

    Lyophilized Mouse G-CSF protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

    Usage notes

    Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse G-CSF protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    Usage notes

    Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse G-CSF protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    Fig

    SDS-PAGE analysis of Mouse G-CSF protein.

    Fig

    SDS-PAGE analysis of Mouse G-CSF protein.

    Note

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

    Note

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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