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重组人干扰素α2a(IFN-α2a)
Human IFN-α2a Protein
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活动截止时间:2024年1月31日
重组人干扰素α2a(IFN-α2a)(货号:PRP1237)是一种由大肠杆菌表达的无标签重组蛋白,基于人源IFN-α2a (Cys24-Glu188)序列(UniProt ID: P01563),分子量为19.4 kDa。该产品以冻干粉形式提供,冻干缓冲液为无菌的20 mM Tris, 150 mM NaCl, pH8.0,专为细胞培养及相关研究应用优化设计。
干扰素α2a(IFN-α2a)属于I型干扰素家族,由IFNA2基因(Gene ID: 83785)编码,位于染色体9的干扰素α基因簇中。作为一种关键的细胞因子,它在病毒感染时被诱导产生,发挥强大的抗病毒、抗肿瘤和免疫调节活性。所有IFN-α亚型共享115-151位氨基酸的保守序列区域,而N端具有可变性,IFN-α2a凭借其高特异性和功能性,成为研究细胞信号传导、免疫应答及疾病机制的理想工具分子。
本产品主要应用于细胞培养、抗病毒机制研究、肿瘤免疫学实验、免疫调节功能评估,以及其他需要高纯度人源细胞因子的生物医学研究领域。
| 中文名称 | 重组人干扰素α2a(IFN-α2a) |
| 英文名称 | Human IFN-α2a Protein |
| 产品货号 | PRP1237 |
| 序列 | 氨基酸序列来源于:人源 IFN-α2a (Cys24-Glu188) (P01563) 表达的蛋白片段。 |
| 标签 | 无标签 |
| 种属 | 人 |
| 活性 | 使用TF-1细胞进行细胞毒性测定。该效应的ED50为0.3301 ng/mL。 |
| 蛋白长度 | 重组人IFN-α2a由165个氨基酸组成,预测分子量为19.4 kDa. |
| 表达宿主 | 大肠杆菌 |
| 纯度 | > 99 % ,使用SDS-PAGE检测 |
| 产品形式 | 冻干粉,冻干缓冲液为无菌的20 mM Tris, 150 mM NaCl, pH8.0. |
| 分子量 | 19.4 kDa |
| 基因ID | 83785 |
| 蛋白质ID | P01563 |
| 蛋白序列链接 | IFNA2 - Interferon alpha-2 - Homo sapiens (Human) | UniProtKB | UniProt |
| 分子 | IFN-α2a |
| 检测指标 | IFN-α2a |
| 注意事项 | 开盖使用前,请先离心。本产品为冻干粉,推荐用该产品配套的Reconstitution Buffer进行复溶,且浓度不低于100 µg/mL。客户可根据后续的实验需求,进行进一步的稀释。 |
| 保存建议 | 本产品为冻干粉形式,建议:将冻干粉产品保存于-20℃以下的干燥环境中。本产品溶解之后,可在4℃保存2-7天,长期使用请置于-20℃以下。为长期保存,推荐添加载体蛋白(0.1% HSA或者BSA),并酌情分装保存,避免反复冻融。 |
| 运输条件 | 冰袋运输(蓝冰) |
| 警告 | 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。 |
Amino acid sequence derived from Human IFN-α2a (P01563) (Cys24-Glu188) was expressed.
The recombinant Human IFN-α2a consists of 165 amino acids and predicts a molecular mass of 19.4 kDa.
Measured by a cytotoxicity assay using TF-1 Cells. The ED50 for this effect is 0.3301 ng/mL.
< 1.0 EU per μg of the protein as determined by the LAL method.
Lyophilized from 20mM Tris, 150mM NaCl, pH 8.0.
Lyophilized protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Lyophilized protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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