SUMO 蛋白酶/Ulp1肽酶(Ulp1)

Sequence

Amino acid sequence derived from Ulp1 isoform (Q02724) ( 403-621 aa + N-terminal Poly-6*His tag C-terminal Poly-6*His tag) was expressed.

Protein length

The recombinant human Ulp1 consists of 219 amino acids and has a predicted molecular mass of 28.7 kDa.

Formulation

Lyophilized from sterile 20 mM Tris, 500 mM NaCl pH 8.0.

Unit Definition

One unit of SUMO Protease is defined as the amount of enzyme needed to cleave 85% of 2 µg of substrate protein at 30°C in one hour.

Enzyme digestion conditions

4°C enzyme digestion overnight is recommended, users can explore according to their own research target protein.After digestion, a small amount of samples can be taken for SDS-PAGE analysis.To remove the Ulp1 in the digested system, use His tag purification resin affinity chromatography.

Storage Instructions

Lyophilized Ulp1 protein product should be stored desiccated below -20°C. Please prevent freeze-thaw cycles.

Fig1. SDS-PAGE analysis of Ulp1.

Usage notes

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Ulp1 to 5 U/μ L using the buffer we provided, which can then be further diluted to other aqueous solutions. The results of the identification of the activity of the produced SUMO Protease enzyme can be referred to the figure.

Reaction System

Fusion Protein

25 μ g

10×SUMO Protease Buffer

5 μ L

SUMO Protease

2 μ L

Total volume

50 μ L

Figure 2. The effect of SUMO Protease on cleaving the target protein with SUMO tag. 25 µg of purified SUMO fusion protein was used for each sample, and the reaction system was 50 uL. 1：SUMO Protease; 2 ：SUMO fusion protein; 3 ：The reaction effect as shown in the above figure; 4-13: The cleavage effects with the enzyme amount halved successively. All reactions were sampled after 1 hour of digestion at 30℃ for SDS-PAGE electrophoresis and Coomassie brilliant blue staining.

(Note:10×SUMO Protease Buffer includes 500 mM Tris, 2% NP-40, 10 mM DTT; pH8.0)

Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.