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重组人白介素-3，His标签（无动物源）（IL-3）是一种重组蛋白产品，由人类胚胎肾细胞表达生产，C端融合多聚组氨酸标签，适用于血清或血浆等生物体液中的细胞因子研究，尤其针对细胞培养应用。该产品以冻干粉形式提供，冻干缓冲液为无菌PBS pH 7.4，便于实验操作。
人源白介素-3（IL-3）是一种具有治疗潜力的关键细胞因子，在免疫系统中参与多种免疫细胞的成熟、分化和功能性激活，主要由活化的T淋巴细胞分泌。与其他细胞因子不同，IL-3具备多谱系潜能和广泛的靶细胞谱，在造血过程中起核心作用；其独特的受体亚基赋予更丰富的功能，已成功应用于改善辐射诱发的骨髓发育不良等疾病研究。CheKine™重组人IL-3蛋白（His标签）提供了一种高特异性工具，通过重组技术确保无动物源成分，支持细胞增殖试验等精确检测。
本产品主要应用于细胞培养、免疫学研究、造血过程分析以及相关治疗开发领域，尤其在细胞因子介导的疾病模型中发挥重要作用。
| 中文名称 | 重组人白介素-3,His-标签(无动物源)(IL-3) |
| 英文名称 | Human IL-3 protein, His tag (Animal-Free) |
| 产品货号 | PRP2015 |
| 序列 | 氨基酸序列来源于：人源 IL-3 蛋白(NP_000579.2，Met1-Phe152) 表达的蛋白片段，C端融合有多聚组氨酸his标签。 |
| 标签 | C-His |
| 种属 | 人 |
| 活性 | 使用TF-1细胞进行细胞增殖试验。该效应的ED50为6.87ng/mL。 |
| 蛋白长度 | 重组人源蛋白IL-3由140个氨基酸组成，预测分子量大小约16kD. |
| 表达宿主 | 人类胚胎肾细胞 |
| 纯度 | > 95 % ，使用SDS-PAGE检测 |
| 产品形式 | 冻干粉，冻干缓冲液为无菌的 PBS，pH 7.4。 |
| 分子量 | 16 kDa |
| 基因ID | 3562 |
| 蛋白质ID | P08700 |
| 蛋白序列链接 | https://www.uniprot.org/uniprot/P08700 |
| 分子 | IL-3 |
| 注意事项 | 开盖使用前，请先离心。本产品为冻干粉，推荐用该产品配套的Reconstitution Buffer进行复溶，且浓度不低于100 µg/mL。客户可根据后续的实验需求，进行进一步的稀释。 |
| 保存建议 | 本产品为冻干粉形式，建议：将冻干粉的重组人白介素-3，His标签（无动物源）（IL-3）产品保存于-20℃以下的干燥环境中。本产品溶解之后，可在4℃保存2-7天，长期使用请置于-20℃以下。为长期保存，推荐添加载体蛋白（0.1% HSA或者BSA），并酌情分装保存，避免反复冻融。 |
| 运输条件 | 常温运输 |
| 警告 | 本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。 |
Amino acid sequence derived from human IL-3 protein (NP_000579.2，Met1-Phe152) was expressed with a polyhistidine tag at the C-terminus.
The recombinant human IL-3 protein consists of 140 amino acids and predicts a molecular mass of 16 kD.The apparent molecular mass of the human IL-3 is approximately 17-35 kDa in SDS-PAGE under reducing conditions due to glycosylation.
The recombinant human IL-3 protein consists of 140 amino acids and predicts a molecular mass of 16 kD.The apparent molecular mass of the human IL-3 is approximately 17-35 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Measured in a cell proliferation assay using TF-1 cells.The ED50 for this effect is 6.87ng/mL.
Measured in a cell proliferation assay using TF-1 cells.The ED50 for this effect is 6.87ng/mL.
< 1 EU per µg of the protein as determined by the LAL method.
< 1 EU per µg of the protein as determined by the LAL method.
Lyophilized from sterile PBS, pH 7.4.
Lyophilized from sterile PBS, pH 7.4.
Lyophilized human IL-3 protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Lyophilized human IL-3 protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized human IL-3 protein in the buffer which we provide not less than 100 µg/mL, which can then be further diluted to other aqueous solutions.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized human IL-3 protein in the buffer which we provide not less than 100 µg/mL, which can then be further diluted to other aqueous solutions.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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