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重组人白介素-12 p70蛋白，His标签（无动物源）（IL-12 p70）是一种通过大肠杆菌表达系统生产的重组细胞因子，用于细胞培养和相关免疫学研究。其氨基酸序列来源于人白介素-12 p70 (Arg23-Ser219) 和 (Ile23-Ser328)，以冻干粉形式提供，冻干缓冲液为无菌PBS (pH 7.4)，确保高稳定性和易用性。
白细胞介素12 p70(IL-12 p70)是一种主要由树突状细胞、巨噬细胞和中性粒细胞等免疫细胞分泌的关键细胞因子，在调节T细胞分化和免疫应答中发挥核心作用。该蛋白由IL-12 p35和IL-12 p40两个亚基组成，分子量约为63-75 kDa，对应基因ID为P29459(p35)和P29460(p40)。本产品重组人IL-12 p70蛋白通过大肠杆菌表达，具有高生物活性，可通过诱导PHA激活的人外周血淋巴细胞(PBMC)分泌IFN-γ的能力验证，其ED50为0.05-0.2 ng/mL，适用于细胞功能研究和免疫学分析。
本产品主要应用于细胞培养实验、免疫调节研究、T细胞激活分析以及肿瘤免疫治疗等生物医药领域。
| 中文名称 | 重组人白介素-12 p70,His-标签(无动物源)(IL-12 p70) |
| 英文名称 | Human IL-12 p70 Protein, His tag (Animal-Free) |
| 产品货号 | PRP1940 |
| 序列 | 氨基酸序列来源于：人白介素-12 p70 (Arg23-Ser219) (P29459), (Ile23-Ser328) (P29460） |
| 种属 | 人 |
| 活性 | 通过其诱导PHA激活的人外周血淋巴细胞(PBMC)分泌IFN-γ的能力来衡量。ED50为0.05-0.2 ng/mL。 |
| 表达宿主 | 大肠杆菌 |
| 纯度 | >98%，使用SDS-PAGE检测 |
| 产品形式 | 冻干粉，冻干缓冲液为无菌的PBS, pH 7.4。 |
| 分子量 | 63-75 kDa |
| 蛋白质ID | P29459(p35), P29460( p40) |
| 分子 | Interleukin-12, NKSF,Cytotoxic Lymphocyte Maturation Factor (CLMF), TSF |
| 检测指标 | Interleukin-12, NKSF,Cytotoxic Lymphocyte Maturation Factor (CLMF), TSF |
| 注意事项 | 开盖使用前，请先离心。本产品为冻干粉，推荐用该产品配套的Reconstitution Buffer进行复溶，且浓度不低于100 µg/mL。客户可根据后续的实验需求，进行进一步的稀释。 |
| 保存建议 | 冻干蛋白应在 -20°C 下保存 1 年。复溶后，在 2°C 至 8°C 下最多可保存 1 周。 在含有载体蛋白或稳定剂的缓冲液（如 0.1% BSA、10%FBS、5%HSA ）中进一步稀释后，分装的蛋白质应在 -20°C 或 -80°C 下保存 3-6 个月。避免反复冻融循环。 |
| 运输条件 | 冰袋运输（蓝冰） |
| 警告 | 本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。 |
Amino acid sequence derived from Human IL-12 p70 (Arg23-Ser219) (P29459), (Ile23-Ser328) (P29460) was expressed with 6×His tag at the C-terminus.
The recombinant Human IL-12 p70 consists of 503 amino acids and predicts a molecular mass of 59.55 kDa.
Measured by its ability to induce IFN gamma secretion in PHA-activated human peripheral blood lymphocytes (PBMC).The ED50 for this effect is 0.05-0.2 ng/mL.
<0.1 EU per 1 μg of the protein by the LAL method.
Lyophilized from PBS, pH 7.4.
Amino acid sequence derived from Human IL-12 p70 (Arg23-Ser219) (P29459), (Ile23-Ser328) (P29460) was expressed with 6×His tag at the C-terminus.
The recombinant Human IL-12 p70 consists of 503 amino acids and predicts a molecular mass of 59.55 kDa.
Measured by its ability to induce IFN gamma secretion in PHA-activated human peripheral blood lymphocytes (PBMC).The ED50 for this effect is 0.05-0.2 ng/mL.
<0.1 EU per 1 μg of the protein by the LAL method.
Lyophilized from PBS, pH 7.4.
Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.
Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Note:暂无相关常见问题。如有疑问，请联系我们的技术支持团队。
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