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重组人半乳糖凝集素8,His-标签(无动物源)(GAL8)
Human Galectin-8 Protein, His tag (Animal-Free)
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活动截止时间:2024年1月31日
                            
                                
                                买2送1活动进行中!购买任意2个规格产品,免费赠送100μL装一支。
活动截止时间:2024年1月31日
重组人半乳糖凝集素8,His-标签(无动物源)(GAL8)是一种在大肠杆菌中表达的高纯度重组蛋白,通过His标签纯化工艺制备,纯度超过98%(SDS-PAGE检测)。产品以冻干粉形式提供,冻干缓冲液为无菌PBS(pH 7.4),并经过活性验证,其凝集人类红细胞的半数有效剂量(ED₅₀)小于8 μg/mL,确保在细胞类研究中提供可靠性能。
半乳糖凝集素-8(Gal-8)属于凝集素家族成员,是一种串联重复型蛋白,包含两个碳水化合物识别结构域(CRD),通过单肽链中的接头区连接。CRD负责特异性结合β-半乳糖苷,其已知结合伴侣包括CD44和特定整联蛋白亚型。Gal-8与整联蛋白形成复合物后,可诱导桩蛋白和粘着斑激酶(FAK)的磷酸化,激活关键信号通路如MAPK-ERK和PI3K-AKT。这一机制使其成为研究细胞粘附、迁移和信号转导的理想工具,特别适用于细胞培养模型中的功能探索。
本产品主要应用于细胞生物学研究领域,特别是在细胞培养系统中用于探索半乳糖凝集素8介导的信号转导、细胞粘附和迁移机制。适用于肿瘤生物学、免疫调节及药物开发等相关研究。
                                    | 中文名称 | 重组人半乳糖凝集素8,His-标签(无动物源)(GAL8) | 
| 英文名称 | Human Galectin-8 Protein, His tag (Animal-Free) | 
| 产品货号 | PRP1928 | 
| 序列 | 氨基酸序列来源于:人半乳糖凝集素8(Met2-Trp317)。 | 
| 种属 | 人 | 
| 活性 | 通过其凝集人类红细胞的能力来测量,其 ED₅₀小于8 μg/mL。 | 
| 表达宿主 | 大肠杆菌 | 
| 纯度 | >98%,使用SDS-PAGE检测 | 
| 产品形式 | 冻干粉,冻干缓冲液为无菌的PBS, pH 7.4。 | 
| 分子量 | 34 kDa | 
| 蛋白质ID | O00214 | 
| 分子 | LGALS8, Gal-8, PCTA-1, PCTA1, Po66-CBP | 
| 检测指标 | LGALS8, Gal-8, PCTA-1, PCTA1, Po66-CBP | 
| 注意事项 | 开盖使用前,请先离心。本产品为冻干粉,推荐用该产品配套的Reconstitution Buffer进行复溶,且浓度不低于100 µg/mL。客户可根据后续的实验需求,进行进一步的稀释。 | 
| 保存建议 | 冻干蛋白应在 -20°C 下保存 1 年。复溶后,在 2°C 至 8°C 下最多可保存 1 周。 在含有载体蛋白或稳定剂的缓冲液(如 0.1% BSA、10%FBS、5%HSA )中进一步稀释后,分装的蛋白质应在 -20°C 或 -80°C 下保存 3-6 个月。避免反复冻融循环。 | 
| 运输条件 | 冰袋运输(蓝冰) | 
| 警告 | 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。 | 
Amino acid sequence derived from Human Galectin-8 (Met2-Trp317) (O00214) was expressed with 6×His tag at the N-terminus.
The recombinant Human Galectin-8 consists of 316 amino acids and predicts a molecular mass of 36.6 kDa.
Amino acid sequence derived from Human Galectin-8 (Met2-Trp317) (O00214) was expressed with 6×His tag at the N-terminus.
The recombinant Human Galectin-8 consists of 316 amino acids and predicts a molecular mass of 36.6 kDa.
Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is <8 μg/mL.
<0.1 EU per 1 μg of the protein by the LAL method.
Lyophilized from PBS, pH 7.4.
Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is <8 μg/mL.
<0.1 EU per 1 μg of the protein by the LAL method.
Lyophilized from PBS, pH 7.4.
Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA, 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA, 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.
SDS-PAGE analysis of Human Galectin-8 protein.
SDS-PAGE analysis of Human Galectin-8 protein.
Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Note:Version 20240115
Abbkine, Inc • USA • www.abbkine.com
Not for further distribution without written consent. Copyright © Abbkine, Inc.
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