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重组小鼠CXC趋化因子配体10,His-标签(无动物源)(CXCL10)
Mouse CXCL10 Protein, His tag (Animal-Free)
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活动截止时间:2024年1月31日
                            
                                
                                买2送1活动进行中!购买任意2个规格产品,免费赠送100μL装一支。
活动截止时间:2024年1月31日
PRP1188 提供的重组小鼠CXC趋化因子配体10蛋白(Mouse CXCL10 Protein, His tag, Animal-Free)是一种在大肠杆菌表达系统中制备、带有His标签的无动物源重组蛋白。该产品以冻干粉形式提供,冻干缓冲液为无菌PBS (pH 7.4),纯度经SDS-PAGE检测超过98%,适用于细胞因子相关研究与细胞培养应用。
CXCL10(又称干扰素γ诱导蛋白10或IP-10)是CXC趋化因子家族成员,由77个氨基酸残基组成,分子量约8.55 kDa。在免疫响应中,CXCL10由单核细胞、内皮细胞等受IFNγ刺激后分泌,对T细胞、NK细胞和巨噬细胞具有特异性趋化作用,是研究炎症、自身免疫疾病及肿瘤微环境的关键介质。本产品通过活性验证,能有效模拟天然趋化功能,为体外细胞迁移和免疫调控实验提供高可靠性蛋白原料。
该蛋白广泛应用于细胞培养体系中的免疫学研究,包括T细胞趋化实验、NK细胞迁移分析、巨噬细胞激活模型以及炎症性疾病机制探索等领域。特别适用于细胞因子信号通路、肿瘤免疫微环境构建及药物筛选等高通量实验场景。
                                    | 中文名称 | 重组小鼠CXC趋化因子配体10,His-标签(无动物源)(CXCL10) | 
| 英文名称 | Mouse CXCL10 Protein, His tag (Animal-Free) | 
| 产品货号 | PRP1188 | 
| 序列 | 氨基酸序列来源于:小鼠CXC趋化因子配体10(Ile22-Pro98)。 | 
| 种属 | 小鼠 | 
| 活性 | 通过其对转染人CXCR3的BaF3细胞的化学吸引能力来衡量,其ED50 < 0.2 μg/mL。 | 
| 表达宿主 | 大肠杆菌 | 
| 纯度 | >98%,使用SDS-PAGE检测 | 
| 产品形式 | 冻干粉,冻干缓冲液为无菌的PBS, pH 7.4 | 
| 分子量 | 11-17 kDa | 
| 蛋白质ID | P17515 | 
| 分子 | IP-10, Gamma-Interferon Inducible Protein 10, Crg-2 | 
| 检测指标 | IP-10, Gamma-Interferon Inducible Protein 10, Crg-2 | 
| 注意事项 | 开盖使用前,请先离心。本产品为冻干粉,推荐用该产品配套的Reconstitution Buffer进行复溶,且浓度不低于100 µg/mL。客户可根据后续的实验需求,进行进一步的稀释。 | 
| 保存建议 | 冻干蛋白应在 -20°C 下保存 1 年。复溶后,在 2°C 至 8°C 下最多可保存 1 周。 在含有载体蛋白或稳定剂的缓冲液(如 0.1% BSA、10%FBS、5%HSA)中进一步稀释后,分装的蛋白质应在 -20°C 或 -80°C 下保存 3-6 个月。避免反复冻融循环。 | 
| 运输条件 | 冰袋运输(蓝冰) | 
| 警告 | 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。 | 
Amino acid sequence derived from Mouse CXCL10 (Ile22-Pro98) (P17515) was expressed with 6×His tag at the N-terminus.
The recombinant Mouse CXCL10 consists of 77 amino acids and predicts a molecular mass of 9.47 kDa.
Measured by its ability to chemoattract BaF3 cells transfected with human CXCR3. The ED50 for this effect is <0.2 μg/mL.
<0.1 EU per 1 μg of the protein by the LAL method.
Lyophilized from PBS, pH 7.4.
Amino acid sequence derived from Mouse CXCL10 (Ile22-Pro98) (P17515) was expressed with 6×His tag at the N-terminus.
The recombinant Mouse CXCL10 consists of 77 amino acids and predicts a molecular mass of 9.47 kDa.
Measured by its ability to chemoattract BaF3 cells transfected with human CXCR3. The ED50 for this effect is <0.2 μg/mL.
<0.1 EU per 1 μg of the protein by the LAL method.
Lyophilized from PBS, pH 7.4.
Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA, 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.
Fig. SDS-PAGE analysis of Mouse CXCL10 protein.
Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Version 20240115
Abbkine, Inc • USA • www.abbkine.com
Not for further distribution without written consent. Copyright © Abbkine, Inc.
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