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重组人干扰素-γ,His-标签(无动物源)(IFN-γ)
Human IFN-γ protein, His tag (Animal-Free)
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活动截止时间:2024年1月31日
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活动截止时间:2024年1月31日
重组人干扰素-γ,His-标签(无动物源)(IFN-γ)是一种在大肠杆菌中表达的重组蛋白,包含C端6×His标签,通过冻干形式提供,适用于细胞培养及相关研究中L-乳酸盐含量检测或其他细胞因子功能分析。
干扰素-γ(IFN-γ)是一种关键细胞因子,属于干扰素家族,主要负责保护细胞免受病毒感染,并在免疫响应中发挥核心作用。研究表明,IFN-γ由抗原激活的免疫细胞(如T细胞、巨噬细胞和树突状细胞)分泌,通过触发巨噬细胞对抗微生物靶标,参与抗增殖、促凋亡和抗肿瘤机制。重组人IFN-γ蛋白(货号:PRP1050)基于人源序列(P01579)表达,其高生物活性(ED50<1 ng/mL,比活力>2×10⁶ IU/mg)确保在HT29细胞等模型中有效诱导细胞毒性,适用于免疫治疗研究和疾病机制探索。
本产品主要应用于细胞培养、免疫学研究、抗病毒机制探索及抗肿瘤药物开发等领域,尤其适合作为细胞因子功能分析的优质试剂。
| 中文名称 | 重组人干扰素-γ,His-标签(无动物源)(IFN-γ) |
| 英文名称 | Human IFN-γ protein, His tag (Animal-Free) |
| 产品货号 | PRP1050 |
| 序列 | 氨基酸序列来源于人源:IFN-γ 蛋白(Gln24-Gln166)(P01579)表达的蛋白片段,在 C 端用 6×His 标签表达。 |
| 标签 | His |
| 种属 | 人 |
| 活性 | 通过其在 HT29 细胞中诱导细胞毒性的能力来衡量。这种效应的ED50<1 ng/mL。重组人 IFN gamma 的比活力约大于2 x 10⁶ IU/mg。 |
| 蛋白长度 | 该蛋白质的计算分子量为 17.7 kDa,在还原条件下,该蛋白质迁移为 17 kDa(SDS-PAGE 分析)。 |
| 表达宿主 | 大肠杆菌 |
| 纯度 | > 95 % ,使用SDS-PAGE检测 |
| 产品形式 | 该蛋白质是由 0.2 µm 过滤溶液冻干而成,其中含有 1X PBS,pH 值为 8.0。如有任何疑问或特殊要求,请与我们联系。 |
| 分子量 | 17.7 kDa |
| 蛋白质ID | P01579 |
| 蛋白序列链接 | http://www.uniprot.org/uniprot/P01579 |
| 分子 | IFN-γ |
| 注意事项 | 开盖使用前,请先离心。本产品为冻干粉,推荐用该产品配套的Reconstitution Buffer进行复溶,且浓度不低于100 µg/mL。客户可根据后续的实验需求,进行进一步的稀释。 |
| 保存建议 | 冻干蛋白应在 -20°C 下保存 1 年。重组后,在 2°C 至 8°C 下最多可保存 1 周。 在含有载体蛋白或稳定剂的缓冲液中进一步稀释(如 0.1%BSA、10%FBS、5%HSA)中进一步稀释,蛋白等分应在 -20°C 或 -80°C 下保存 3-6 个月。避免反复冻融循环。 |
| 运输条件 | 冰袋运输(蓝冰) |
| 警告 | 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。 |
Amino acid sequence derived from Human IFN-γ protein (Gln24-Gln166)(P01579) was expressed with 6×His tag at the C-terminus.
The secreted recombinant Human IFN-γ consists of 143 amino acids and has a predicted molecular mass of 17.7 kDa.
The secreted recombinant Human IFN-γ consists of 143 amino acids and has a predicted molecular mass of 17.7 kDa.
Measure by its ability to induce cytotoxicity in HT29 cells. The ED50 for this effect is <1 ng/mL. The specific activity of recombinant Human IFN-γ is approximately >2 x 10⁶ IU/mg.
Measure by its ability to induce cytotoxicity in HT29 cells. The ED50 for this effect is <1 ng/mL. The specific activity of recombinant Human IFN-γ is approximately >2 x 10⁶ IU/mg.
< 0.01 EU per μg of the protein as determined by the LAL method.
< 0.01 EU per μg of the protein as determined by the LAL method.
Lyophilized from sterile PBS, pH 8.0.
Lyophilized from sterile PBS, pH 8.0.
Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA, 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA, 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Human IFN-γ protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Human IFN-γ protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.
Fig. SDS-PAGE analysis of Human IFN-γ protein.
Fig. SDS-PAGE analysis of Human IFN-γ protein.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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