重组人B细胞激活因子(BAFF)

Name: 重组人B细胞激活因子(BAFF)
Brand: Abbkine
SKU: PRP1035
Price: 588 CNY
Availability: InStock

Human BAFF Protein

产品货号

PRP1035

产品特点：

高纯度保证：>99%纯度，经SDS-PAGE严格验证，确保实验一致性

低内毒素水平：采用LAL法检测，每1μg蛋白质中EU含量小于0.1，减少细胞毒性干扰

无标签设计：避免额外标签对蛋白功能的影响，维持天然结合活性

功能性验证：通过BCMA结合实验（EC50=0.2 μg/mL）确认高生物活性

优化表达系统：大肠杆菌宿主高效表达，支持稳定生产和快速应用

选择规格

10 μg

¥588

现货（次日发货）

50 µg

¥2198

现货（次日发货）

1 mg

¥19898

请咨询

🎉 限时优惠

买2送1活动进行中！购买任意2个规格产品，免费赠送100μL装一支。

活动截止时间：2024年1月31日

产品概述

重组人B细胞激活因子（BAFF）蛋白（Human BAFF Protein）是一种重组细胞因子，通过功能性ELISA方法高灵敏度检测其与配体（如BCMA）的结合活性，适用于细胞培养、免疫学研究和B细胞相关功能分析等领域。

B细胞激活因子（BAFF），又称TNFSF13B或CD257，是一种肿瘤坏死因子家族成员，主要表达于外周血白细胞（特别是单核细胞和巨噬细胞）。它通过与受体TACI、BCMA和BAFFR结合，在B细胞分化、稳定和选择中发挥关键调控作用。本产品基于功能性ELISA原理，包被1 μg/mL（100μl/孔）人BAFF后，可高效结合人BCMA(C-mFc)，其EC50为0.2 μg/mL，为B细胞信号通路研究提供精准、可靠的检测工具。

本产品广泛应用于细胞培养体系、免疫学研究、B细胞分化机制探索及自身免疫性疾病模型开发等前沿领域。

产品参数

中文名称	重组人B细胞激活因子(BAFF)
英文名称	Human BAFF Protein
产品货号	PRP1035
序列	氨基酸序列来源于：人源BAF (Q9Y275-1) (Ala 134-Leu 285)表达的蛋白片段。
标签	无标签
种属	人
活性	检测其与配体的结合能力，检测的实验类型为功能性ELISA。包被的1 μg/mL(100μl/孔)人BAFF可与人BCMA(C-mFc)结合，人BCMA(C-mFc)的EC50为0.2 μg/mL。
蛋白长度	重组人SHH由152个氨基酸组成，在N端有6×His标签，预测分子质量为22.9 kDa。
表达宿主	大肠杆菌
纯度	> 99 % ，使用SDS-PAGE检测
产品形式	冻干粉，冻干缓冲液为无菌的 PBS，pH 7.5。
分子量	22.9 kDa
基因ID	10673
蛋白质ID	Q96RJ3
蛋白序列链接	https://www.uniprot.org/uniprot/Q96RJ3
分子	BAFF
注意事项	开盖使用前，请先离心。本产品为冻干粉，推荐用该产品配套的Reconstitution Buffer进行复溶
保存建议	本产品为冻干粉形式，建议：产品保存于-20℃以下的干燥环境中，溶解之后，可在4℃保存2-7天，长期使用请置于-20℃以下。
运输条件	常温运输
警告	本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。

使用说明

Technical Specifications

Sequence:

Amino acid sequence derived from Human BAFF (Q9Y275-1) (Ala 134-Leu 285) was expressed.

Protein length:

The recombinant Human BAFF consists of 152 amino acids with 6×His tag at N terminus and predicts a molecular mass of 22.9 kDa.

Biological Activity:

Immobilized Human BAFF at 1 μg/mL (100μl/well) can bind Human BCMA (C-mFc), The EC50 of Human BCMA is 0.2 μg/mL.

Endotoxin:

< 1.0 EU per μg of the protein as determined by the LAL method.

Formulation:

Lyophilized from PBS, pH 7.5.

Sequence:

Amino acid sequence derived from Human BAFF (Q9Y275-1) (Ala 134-Leu 285) was expressed.

Sequence:

Amino acid sequence derived from Human BAFF (Q9Y275-1) (Ala 134-Leu 285) was expressed.

Protein length:

The recombinant Human BAFF consists of 152 amino acids with 6×His tag at N terminus and predicts a molecular mass of 22.9 kDa.

Protein length:

The recombinant Human BAFF consists of 152 amino acids with 6×His tag at N terminus and predicts a molecular mass of 22.9 kDa.

Biological Activity:

Immobilized Human BAFF at 1 μg/mL (100μl/well) can bind Human BCMA (C-mFc), The EC50 of Human BCMA is 0.2 μg/mL.

Biological Activity:

Immobilized Human BAFF at 1 μg/mL (100μl/well) can bind Human BCMA (C-mFc), The EC50 of Human BCMA is 0.2 μg/mL.

Endotoxin:

< 1.0 EU per μg of the protein as determined by the LAL method.

Endotoxin:

< 1.0 EU per μg of the protein as determined by the LAL method.

Formulation:

Lyophilized from PBS, pH 7.5.

Formulation:

Lyophilized from PBS, pH 7.5.

Storage Instructions

Lyophilized recombinant Human BAFF protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Usage Notes

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Human BAFF protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Fig. SDS-PAGE analysis of Human BAFF protein.

Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Note: