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重组人白介素3(IL-3)

Human IL-3 Protein

产品货号
PRP1034

产品特点:

  • 高纯度:> 96 %,经SDS-PAGE严格检测,确保实验可靠性。
  • 高活性:ED50为0.2575 ng/mL,在TF-1细胞增殖模型中验证效能。
  • 低内毒素:采用LAL法检测,每1 μg蛋白质中EU含量<1.0,减少干扰因素。
  • 无标签设计:避免额外修饰,适用于敏感细胞实验和下游应用。
  • 冻干粉形式:缓冲液为无菌PBS pH 7.4,便于复溶和储存管理。
  • 选择规格

    20 µg
    ¥1498
    现货(次日发货)
    100 µg
    ¥4698
    现货(次日发货)
    1 mg
    ¥14998
    请咨询
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    活动截止时间:2024年1月31日

    产品概述

    重组人白介素3(IL-3)蛋白是一种由大肠杆菌表达系统生产的无标签重组蛋白,通过其在TF-1细胞中诱导增殖的高灵敏度活性(ED50为0.2575 ng/mL),用于细胞培养、免疫学研究及生物样本分析的高纯度工具。

    IL-3(白细胞介素3)是一种关键的促进生长细胞因子,属于白介素家族,在免疫系统中发挥核心作用。它由多种体细胞产生,通过刺激造血细胞的增殖和分化来维持免疫稳态;IL-3功能缺失与自身免疫性疾病或免疫缺陷相关。重组人IL-3蛋白基于人源序列(NP_000579.2, Ala20-Phe152)表达,提供了一种可靠的研究工具,适用于探索细胞信号通路、免疫调节机制及疾病模型构建。

    本产品主要应用于细胞培养领域,特别适用于免疫学、血液学及药物开发研究,用于探究细胞因子介导的增殖、分化过程,以及相关疾病机制的体外模型建立。

    重组人白介素3(IL-3)

    产品参数

    中文名称 重组人白介素3(IL-3)
    英文名称 Human IL-3 Protein
    产品货号 PRP1034
    序列 氨基酸序列来源于:人源IL-3 (NP_000579.2) (Ala20-Phe152)表达的蛋白片段。
    标签 无标签
    种属
    活性 通过其在TF-1细胞中诱导增殖的能力来测定。该效应的ED50为0.2575 ng/mL。
    蛋白长度 重组人IL-3由133个氨基酸组成,预测分子质量为15.2 kDa。
    表达宿主 大肠杆菌
    纯度 > 96 % ,使用SDS-PAGE检测
    产品形式 冻干粉,冻干缓冲液为无菌的 PBS,pH 7.4。
    分子量 15.2 kDa
    基因ID 3562
    蛋白质ID P08700
    蛋白序列链接 https://www.uniprot.org/uniprot/P08700
    分子 IL-3
    注意事项 开盖使用前,请先离心。本产品为冻干粉,推荐用该产品配套的Reconstitution Buffer进行复溶
    保存建议 本产品为冻干粉形式,建议:产品保存于-20℃以下的干燥环境中,溶解之后,可在4℃保存2-7天,长期使用请置于-20℃以下。
    运输条件 冰袋运输(蓝冰)
    警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

    使用说明

    Sequence

    Amino acid sequence derived from Human IL-3 (NP_000579.2) (Ala20-Phe152) was expressed with an initial Met.

    Protein length

    The recombinant Human IL-3 consists of 133 amino acids and has a predicted molecular mass of 15.2 kDa.

    Protein length

    The recombinant Human IL-3 consists of 133 amino acids and has a predicted molecular mass of 15.2 kDa.

    Biological Activity

    Measured by its ability to induce proliferation in TF-1 cells. The ED50 for this effect is 0.2575 ng/mL.

    Biological Activity

    Measured by its ability to induce proliferation in TF-1 cells. The ED50 for this effect is 0.2575 ng/mL.

    Endotoxin

    < 0.1 EU per μg of the protein as determined by the LAL method.

    Endotoxin

    < 0.1 EU per μg of the protein as determined by the LAL method.

    Formulation

    Lyophilized from sterile PBS, pH 7.4.

    Formulation

    Lyophilized from sterile PBS, pH 7.4.

    Storage Instructions

    Lyophilized Human IL-3 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

    Storage Instructions

    Lyophilized Human IL-3 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

    Usage notes

    Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL-3 protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    Usage notes

    Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL-3 protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    Fig. SDS-PAGE analysis of Human IL-3 protein.

    Fig. SDS-PAGE analysis of Human IL-3 protein.

    Note

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

    Note

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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