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重组人白介素3(IL-3)
Human IL-3 Protein
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活动截止时间:2024年1月31日
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活动截止时间:2024年1月31日
重组人白介素3(IL-3)蛋白是一种由大肠杆菌表达系统生产的无标签重组蛋白,通过其在TF-1细胞中诱导增殖的高灵敏度活性(ED50为0.2575 ng/mL),用于细胞培养、免疫学研究及生物样本分析的高纯度工具。
IL-3(白细胞介素3)是一种关键的促进生长细胞因子,属于白介素家族,在免疫系统中发挥核心作用。它由多种体细胞产生,通过刺激造血细胞的增殖和分化来维持免疫稳态;IL-3功能缺失与自身免疫性疾病或免疫缺陷相关。重组人IL-3蛋白基于人源序列(NP_000579.2, Ala20-Phe152)表达,提供了一种可靠的研究工具,适用于探索细胞信号通路、免疫调节机制及疾病模型构建。
本产品主要应用于细胞培养领域,特别适用于免疫学、血液学及药物开发研究,用于探究细胞因子介导的增殖、分化过程,以及相关疾病机制的体外模型建立。
| 中文名称 | 重组人白介素3(IL-3) |
| 英文名称 | Human IL-3 Protein |
| 产品货号 | PRP1034 |
| 序列 | 氨基酸序列来源于:人源IL-3 (NP_000579.2) (Ala20-Phe152)表达的蛋白片段。 |
| 标签 | 无标签 |
| 种属 | 人 |
| 活性 | 通过其在TF-1细胞中诱导增殖的能力来测定。该效应的ED50为0.2575 ng/mL。 |
| 蛋白长度 | 重组人IL-3由133个氨基酸组成,预测分子质量为15.2 kDa。 |
| 表达宿主 | 大肠杆菌 |
| 纯度 | > 96 % ,使用SDS-PAGE检测 |
| 产品形式 | 冻干粉,冻干缓冲液为无菌的 PBS,pH 7.4。 |
| 分子量 | 15.2 kDa |
| 基因ID | 3562 |
| 蛋白质ID | P08700 |
| 蛋白序列链接 | https://www.uniprot.org/uniprot/P08700 |
| 分子 | IL-3 |
| 注意事项 | 开盖使用前,请先离心。本产品为冻干粉,推荐用该产品配套的Reconstitution Buffer进行复溶 |
| 保存建议 | 本产品为冻干粉形式,建议:产品保存于-20℃以下的干燥环境中,溶解之后,可在4℃保存2-7天,长期使用请置于-20℃以下。 |
| 运输条件 | 冰袋运输(蓝冰) |
| 警告 | 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。 |
Amino acid sequence derived from Human IL-3 (NP_000579.2) (Ala20-Phe152) was expressed with an initial Met.
The recombinant Human IL-3 consists of 133 amino acids and has a predicted molecular mass of 15.2 kDa.
The recombinant Human IL-3 consists of 133 amino acids and has a predicted molecular mass of 15.2 kDa.
Measured by its ability to induce proliferation in TF-1 cells. The ED50 for this effect is 0.2575 ng/mL.
Measured by its ability to induce proliferation in TF-1 cells. The ED50 for this effect is 0.2575 ng/mL.
< 0.1 EU per μg of the protein as determined by the LAL method.
< 0.1 EU per μg of the protein as determined by the LAL method.
Lyophilized from sterile PBS, pH 7.4.
Lyophilized from sterile PBS, pH 7.4.
Lyophilized Human IL-3 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Lyophilized Human IL-3 protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL-3 protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL-3 protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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