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重组小鼠转化生长因子-β1,His-标签(无动物源)(TGF-β1)

Mouse TGF-β1 protein, His tag (Animal-Free)

产品货号
PRP1017

产品特点:

  • 高纯度保障:纯度 >95%,经SDS-PAGE严格检测,确保实验可靠性
  • 低内毒素水平:采用LAL法检测,每1 μg蛋白质中EU含量小于1.0,减少背景干扰
  • 无动物源生产:在人类胚胎肾细胞中表达,避免动物源成分污染,提高生物相容性
  • 优化储存形式:冻干粉设计,便于长期保存于-20℃以下干燥环境,复溶后灵活使用
  • 选择规格

    10 μg
    ¥588
    现货(次日发货)
    50 μg
    ¥1498
    现货(次日发货)
    100 μg
    ¥1816
    现货(次日发货)
    1 mg
    ¥请咨询
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    活动截止时间:2024年1月31日

    产品概述

    重组小鼠转化生长因子-β1,His标签(无动物源)(TGF-β1)是一种在人类胚胎肾细胞中表达的重组蛋白,包含C端多聚组氨酸标签,由367个氨基酸组成,预测分子量为42.7 kDa。本品以冻干粉形式提供,冻干缓冲液为无菌PBS(pH 7.4),适用于细胞类样本的研究和分析。

    TGF-β1是转化生长因子β(TGF-β)家族的关键成员,参与调控多种细胞过程,包括细胞分裂、分化、运动、粘附和死亡。它在免疫系统中发挥双重作用:一方面抑制干扰素-γ、肿瘤坏死因子-α等细胞因子的分泌,降低受体表达;另一方面促进T细胞增殖并调节B细胞和骨髓细胞功能。在骨生物学中,TGF-β1刺激成骨细胞骨形成,驱动趋化性、增殖和分化。此外,其在肿瘤发生中扮演复杂角色,自分泌信号可促进肿瘤进展,晚期癌症中水平升高常与疾病侵袭性相关。本品提供高纯度重组小鼠源TGF-β1蛋白,支持精准的细胞调控机制研究。

    本产品主要应用于细胞培养领域,包括细胞增殖与分化调控研究、免疫细胞(如T细胞和B细胞)功能分析、癌症机制探索以及骨重塑相关实验。

    重组小鼠转化生长因子-β1,His-标签(无动物源)(TGF-β1)

    产品参数

    中文名称 重组小鼠转化生长因子-β1,His-标签(无动物源)(TGF-β1)
    英文名称 Mouse TGF-β1 protein, His tag (Animal-Free)
    产品货号 PRP1017
    序列 氨基酸序列来源于:鼠源 TGF-β1(P04202)(Met1-Ser390) 表达的蛋白片段,C端融合有多聚组氨酸标签。
    标签 C-His
    种属 小鼠
    蛋白长度 重组小鼠TGF-β1由367个氨基酸组成,预测分子量为42.7 KD。在还原性条件下的SDS-PAGE中,重组小鼠TGF beta 1的分子量约为54、41、15kDa。
    表达宿主 人类胚胎肾细胞
    纯度 > 95 % ,使用SDS-PAGE检测
    产品形式 冻干粉,冻干缓冲液为无菌的 PBS,pH 7.4。
    分子量 42.7 kDa
    基因ID 21803
    蛋白质ID P04202
    蛋白序列链接 https://www.uniprot.org/uniprot/P04202
    分子 TGF-β1
    注意事项 开盖使用前,请先离心。本产品为冻干粉,推荐用该产品配套的Reconstitution Buffer进行复溶,且浓度不低于100 µg/mL。客户可根据后续的实验需求,进行进一步的稀释。
    保存建议 本产品为冻干粉形式,建议:将冻干粉的重组小鼠转化生长因子-β1,His标签(无动物源)(TGF-β1)产品保存于-20℃以下的干燥环境中。本产品溶解之后,可在4℃保存2-7天,长期使用请置于-20℃以下。为长期保存,推荐添加载体蛋白(0.1% HSA或者BSA),并酌情分装保存,避免反复冻融。
    运输条件 冰袋运输(蓝冰)
    警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

    使用说明

    Sequence

    Amino acid sequence derived from mouse TGF-β1 (P04202) (Met1-Ser390) was expressed with a 6 His tag at the C-terminus.

    Protein length

    The recombinant mouse TGF-β1 consists of 367 amino acids and predicts a molecular mass of 42.7 kDa. It migrates as an approximately 54, 41 and 15 kDa band in SDS-PAGE under reducing conditions.

    Protein length

    The recombinant mouse TGF-β1 consists of 367 amino acids and predicts a molecular mass of 42.7 kDa. It migrates as an approximately 54, 41 and 15 kDa band in SDS-PAGE under reducing conditions.

    Biological Activity

    Testing in progress.

    Biological Activity

    Testing in progress.

    Endotoxin

    < 1.0 EU per μg of the protein as determined by the LAL method.

    Endotoxin

    < 1.0 EU per μg of the protein as determined by the LAL method.

    Formulation

    Lyophilized from sterile PBS, pH 7.4.

    Formulation

    Lyophilized from sterile PBS, pH 7.4.

    Storage Instructions

    Lyophilized Mouse TGF-β1 protein product should be stored desiccated below -18 ℃ . Upon reconstitution, the protein should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

    Storage Instructions

    Lyophilized Mouse TGF-β1 protein product should be stored desiccated below -18 ℃ . Upon reconstitution, the protein should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

    Usage notes

    Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse TGF-β1 protein with the buffer we provided not less than 100μg/ml, which can then be further diluted to other aqueous solutions.

    Usage notes

    Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse TGF-β1 protein with the buffer we provided not less than 100μg/ml, which can then be further diluted to other aqueous solutions.

    Fig. SDS-PAGE analysis of Mouse TGF-β1 protein.

    Disclaimer

    Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

    Disclaimer

    Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

    Version 20240914
    Abbkine, Inc • USA • www.abbkine.com

    常见问题

    暂无相关常见问题。如有疑问,请联系我们的技术支持团队。

    文献引用

    Overexpression of fibroblast growth factor receptor 2 in bone marrow mesenchymal stem cells enhances osteogenesis and promotes critical cranial bone …

    杂志名称: Frontiers in Cell and Developmental Biology | 作者: Zhou, Yiwen, et al.

    IF: 6 | 发表时间: 2023

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