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重组小鼠干扰素-γ,His-标签(IFN-γ)
Mouse IFN-γ protein, His Tag
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活动截止时间:2024年1月31日
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活动截止时间:2024年1月31日
PRP1015 重组小鼠干扰素-γ,His-标签(IFN-γ)是一种在大肠杆菌中表达的重组蛋白,基于鼠源 γ-IFN (NP_P01580) 序列(His23-Cys155),N端融合6xHis标签,预测分子量为16.9 kDa,纯度>95%,以冻干粉形式提供,适用于细胞培养等研究中细胞因子的功能分析。
干扰素-γ(IFN-γ)是一种由淋巴细胞产生的细胞因子,通过结合细胞表面受体激活抗病毒蛋白合成,抑制病毒复制,同时增强自然杀伤细胞(NK细胞)、巨噬细胞和T淋巴细胞的活性,发挥免疫调节作用。本产品通过功能性ELISA验证其与配体(如小鼠IFNGR1)的结合能力,EC50为144 ng/mL;在TF-1细胞毒性测定中,ED50为0.03279 ng/mL,适用于细胞功能研究。
本产品主要应用于细胞培养、免疫调节机制研究、抗病毒药物筛选及功能性检测(如ELISA和细胞毒性实验)等领域。
| 中文名称 | 重组小鼠干扰素-γ,His-标签(IFN-γ) |
| 英文名称 | Mouse IFN-γ protein, His Tag |
| 产品货号 | PRP1015 |
| 序列 | 氨基酸序列来源于:鼠源 γ-IFN (NP_P01580) (His23-Cys155)表达的蛋白片段,N端含有6xHis标签。 |
| 标签 | N-His |
| 种属 | 小鼠 |
| 活性 | 检测其与配体的结合能力,检测的实验类型为功能性ELISA。1 μg/mL的包被小鼠IFN-γ蛋白(100 μl/孔)可与小鼠IFNGR1结合,小鼠IFNGR1的EC50为144ng/mL。采用TF-1细胞进行细胞毒性测定。该效应的ED50为0.03279 ng/mL。 |
| 蛋白长度 | 重组小鼠 IFN-γ由154个氨基酸组成,预测的理论分子量为16.9 KDa,与SDS-PAGE结果一致。 |
| 表达宿主 | 大肠杆菌 |
| 纯度 | > 95 % ,使用SDS-PAGE检测 |
| 产品形式 | 冻干粉,冻干缓冲液为无菌的 150mM NaCl 20mM Tris , pH 8.0溶液。 |
| 分子量 | 16.9 kDa |
| 基因ID | 15978 |
| 蛋白质ID | P01580 |
| 蛋白序列链接 | http://www.uniprot.org/uniprot/P01580 |
| 分子 | IFN-γ |
| 注意事项 | 开盖使用前,请先离心。本产品为冻干粉,推荐用该产品配套的Reconstitution Buffer进行复溶,且浓度不低于100 µg/mL。客户可根据后续的实验需求,进行进一步的稀释。 |
| 保存建议 | 本产品为冻干粉形式,建议:将冻干粉的重组小鼠干扰素-γ,His标签(IFN-γ)产品保存于-20℃以下的干燥环境中。本产品溶解之后,可在4℃保存2-7天,长期使用请置于-20℃以下。为长期保存,推荐添加载体蛋白(0.1% HSA或者BSA),并酌情分装保存,避免反复冻融。 |
| 运输条件 | 常温运输 |
| 警告 | 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。 |
Amino acid sequence derived from Mouse IFN-γ protein soluble form (NP_P01580) (His23-Cys155) was expressed, was expressed with a polyhistidine tag at the N-terminus.
The recombinant Mouse IFN-γ protein consists of 154 amino acids and predicts a molecular mass of 16.9 kDa.
The recombinant Mouse IFN-γ protein consists of 154 amino acids and predicts a molecular mass of 16.9 kDa.
Immobilized Mouse IFN-γ protein at 1 μg/mL (100 μl/well) can bind Mouse IFNGR1, The EC50 of Mouse IFNGR1 is 144 ng/mL;Measured by a cytotoxicity assay using TF-1 Cells. The ED50 for this effect is 0.03279 ng/mL ng/mL.
Immobilized Mouse IFN-γ protein at 1 μg/mL (100 μl/well) can bind Mouse IFNGR1, The EC50 of Mouse IFNGR1 is 144 ng/mL;Measured by a cytotoxicity assay using TF-1 Cells. The ED50 for this effect is 0.03279 ng/mL ng/mL.
Lyophilized from sterile 150mM NaCl 20mM Tris , pH 8.0.
Lyophilized from sterile 150mM NaCl 20mM Tris , pH 8.0.
Lyophilized Mouse IFN-γ protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Lyophilized Mouse IFN-γ protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse IFN-γ protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse IFN-γ protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
暂无相关常见问题。如有疑问,请联系我们的技术支持团队。
杂志名称: Cells | 作者: Luo J, Wang J, Zhang J, et al.
IF: 8 | 发表时间: 2022
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