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重组人转化生长因子-β1(TGF-β1)
Human TGF-β1 protein
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活动截止时间:2024年1月31日
重组人转化生长因子-β1(TGF-β1)是一种基于中国仓鼠卵巢细胞(CHO)稳转表达系统生产的重组蛋白,通过高效纯化工艺制备,用于细胞培养等研究中调控细胞生长、分化、凋亡及免疫反应的功能性细胞因子。
转化生长因子-β(TGF-β)属于多功能蛋白质超家族,包含TGF-β1、TGF-β2和TGF-β3等亚型。其中,人源TGF-β1(蛋白质ID:P01137,基因ID:7040)通过结合细胞表面丝氨酸/苏氨酸激酶受体,激活SMAD或DAXX信号通路,实现对细胞增殖、分化、凋亡及免疫调节的精准调控。本产品通过检测其对貂肺上皮细胞(Mv-1-lu)增殖的抑制能力进行活性验证,ED50为0.04-0.2 ng/ml,确保其在细胞实验中的高效性和可靠性。
本产品广泛应用于细胞培养优化、干细胞分化研究、免疫调节实验、细胞信号通路分析及组织工程等生物医学领域。
                                    | 中文名称 | 重组人转化生长因子-β1(TGF-β1) | 
| 英文名称 | Human TGF-β1 protein | 
| 产品货号 | PRP100190 | 
| 序列 | 氨基酸序列来源于: active form of人源/rhesus/canine TGFβ1 (NP_000651.3)。(Ala 279-Ser 390)表达的蛋白片段。人源, 恒河猴和犬序列相同。 | 
| 标签 | 无标签 | 
| 种属 | 人 | 
| 活性 | 检测其抑制貂肺上皮细胞(Mv-1-lu)增殖的能力,ED50 为 0.04-0.2 ng/ml。 | 
| 蛋白长度 | 重组人/猕猴/犬类的TGF-β1由112个氨基酸组成,理论分子量为12.8 KDa。在还原性和非还原性SDS-PAGE中显示的条带分别为13和26 KDa,对应的是它的单体和同源二聚体形式。 | 
| 表达宿主 | 中国仓鼠卵巢细胞稳转细胞 | 
| 纯度 | > 95 % ,使用HPLC检测. | 
| 产品形式 | 液体,缓冲液为无菌的 100mM GLY, 10mM NaCl, pH 3.0溶液 | 
| 分子量 | 12.8 kDa | 
| 基因ID | 7040 | 
| 蛋白质ID | P01137 | 
| 蛋白序列链接 | http://www.uniprot.org/uniprot/P01137 | 
| 分子 | TGF-β1 | 
| 注意事项 | 客户可根据后续的实验需求,进行进一步的稀释。 | 
| 保存建议 | 本产品为液体形式,建议:将冻干粉的重组人转化生长因子-β1(TGF-β1)产品保存于-20℃以下的干燥环境中。可在4℃保存2-7天,长期使用请置于-20℃以下。为长期保存,推荐添加载体蛋白(0.1% HSA或者BSA),并酌情分装保存,避免反复冻融。 | 
| 运输条件 | 常温运输 | 
| 其它 | 每1ug细胞因子中,内毒素含量低于0.1 EU(LAL法测定) | 
| 警告 | 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。 | 
Amino acid sequence derived from active form of human/rhesus/canine TGFβ1 (NP_000651.3) (Ala 279-Ser 390) was expressed and purified. Human, Rhesus and Canine TGFβ1 sequences are identical.
The recombinant human/rhesus/canine TGFβ1 consists of 112 amino acids and has a calculated molecular mass of 12.8 kDa. it migrates as an approximately 13 & 26 kDa band in reduced and non-reduced SDS-PAGE respectively, corresponding to the monomer and homodimer.
The recombinant human/rhesus/canine TGFβ1 consists of 112 amino acids and has a calculated molecular mass of 12.8 kDa. it migrates as an approximately 13 & 26 kDa band in reduced and non-reduced SDS-PAGE respectively, corresponding to the monomer and homodimer.
Liquid in from sterile 100mM GLY, 10mM NaCl, pH 3.0.
Liquid in from sterile 100mM GLY, 10mM NaCl, pH 3.0.
Lyophilized Human TGF-β1 protein product should be stored desiccated below -20°C. The protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Lyophilized Human TGF-β1 protein product should be stored desiccated below -20°C. The protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Always centrifuge tubes before opening. It is recommended to diluted the protein using the PBS buffer or Protein storage solution to working concentration.
Always centrifuge tubes before opening. It is recommended to diluted the protein using the PBS buffer or Protein storage solution to working concentration.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
A: 由于复溶buffer中40%acetonitrile极易升华,最后试剂的pH发生变化而导致了蛋白最终的析出。如有析出,建议客户用30mM乙酸或者4mM盐酸的话,可以用少量体积做复溶尝试,如果沉淀可逆则可以正常使用。
杂志名称: Plos one | 作者: Payuhakrit, Witchuda, et al.
IF: 3 | 发表时间: 2024
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