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植物Actin小鼠单克隆抗体(3T3)

Anti-Plant Actin Mouse Monoclonal Antibody (3T3)

产品货号
ABL1050

产品特点:

  • 小鼠IgG1亚型单克隆抗体,批次间差异小,信号稳定
  • 经亲和层析纯化,有效去除非特异成分,背景更低
  • 预稀释液体形式,开盖即用,节省实验准备时间
  • 兼容拟南芥、玉米、油菜等多种植物样本,适用性广
  • 43 kDa目标条带清晰,适合作为植物WB内参
  • 选择规格

    50 μL
    ¥498
    现货(当天发货)
    200 μL
    ¥898
    现货(当天发货)
    200 μL×5
    ¥3998
    现货(次日发货)
    🎉 限时优惠

    买2送1活动进行中!购买任意2个规格产品,免费赠送100μL装一支。

    活动截止时间:2024年1月31日

    产品概述

    亚科因生物研发的植物Actin小鼠单克隆抗体(3T3)(Anti-Plant Actin Mouse Monoclonal Antibody, 3T3 clone)是一款专为植物样本设计的内参抗体,可在Western Blot实验中精准识别并标记43 kDa的植物Actin蛋白,为蛋白表达量归一化提供可靠参照。

    Actin是真核生物细胞骨架的核心组分,在植物体内高度保守,广泛参与胞质流动、细胞形态建成、细胞分裂、细胞器运动及生长延伸等关键生理过程。该蛋白在幼嫩及快速扩张的组织、花器官原基、发育中的种子及新生花序中表达尤为丰富。拟南芥Actin基因家族复杂,其中ACT2与ACT8同属一类,蛋白序列相似但侧翼序列、内含子及同义密码子位点存在差异;ACT8在根、茎、叶、花、花粉及角果中均有表达。本抗体以重组植物Actin蛋白为免疫原,经小鼠单克隆技术制备,可特异性识别拟南芥、玉米及油菜中的Actin,确保WB结果的高特异与高重现。

    应用领域

    主要用于植物分子生物学研究中的Western Blot内参检测,适用于基因表达分析、蛋白定量比较、胁迫响应及发育生物学等实验场景。

    植物Actin小鼠单克隆抗体(3T3)

    产品参数

    中文名称 植物Actin小鼠单克隆抗体(3T3)
    英文名称 Anti-Plant Actin Mouse Monoclonal Antibody (3T3)
    产品货号 ABL1050
    免疫原 重组蛋白
    宿主 小鼠
    反应性 拟南芥#玉米#油菜
    检测类型 WB
    克隆类型 单克隆
    亚型 小鼠IgG1
    纯化工艺 通过使用特异性免疫原的亲和层析,从小鼠腹水中纯化抗体。
    产品形式 液体形式
    基因ID 820385
    蛋白质ID P53496
    目标条带分子量 43
    蛋白序列链接 http://www.uniprot.org/uniprot/P53496
    分子 Actin
    储存缓冲液 PBS缓冲液(pH 7.4),0.02%叠氮化钠(防腐剂)和50%甘油。
    保存建议 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
    运输条件 冰袋运输(蓝冰)
    警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

    使用说明

    Application Notes

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000-1:10000).

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000-1:10000).

    Storage Buffer

    Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.

    Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.

    Storage Instructions

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

    Western Blot Analysis

    Western blot analysis of plant Actin expression in Arabidopsis (lane 1-4) with Anti-Plant Actin Mouse Monoclonal Antibody (3T3) (ABL1050, 1:2000) and HRP, Goat Anti-Mouse IgG (A21010, 1:10000).

    Western blot analysis of plant Actin expression in Arabidopsis (lane 1-4) with Anti-Plant Actin Mouse Monoclonal Antibody (3T3) (ABL1050, 1:2000) and HRP, Goat Anti-Mouse IgG (A21010, 1:10000).

    Disclaimer

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

    常见问题

    Q: 普通荧光二抗能不能做WB呢?应该如何成像呢?

    A: 荧光western blotting的结果需要特殊的仪器检测,即一种荧光成像仪器。即运用荧光成像仪可以对荧光WB的实验结果进行成像和数据采集分析。如果希望应用荧光成像做WB,建议选用Abbkine DyLight 680和DyLight 800荧光二抗,货号分别为A23710、A23720、A23910、A23920。

    Q: Abbkine品牌的二抗的浓度为多少呢?

    A: 0.5 mg/ml。

    Q: 抗体中甘油的作用是什么?

    A: 甘油是可以减慢抗体分子之间的碰撞,起到抗体保护剂的作用。

    文献引用

    A tripartite rheostat controls self-regulated host plant resistance to insects

    杂志名称: Nature | 作者: Guo, Jianping, et al.

    IF: 65 | 发表时间: 2023

    Pathogenesis-related protein 1 suppresses oomycete pathogen by targeting against AMPK kinase complex

    杂志名称: Journal of Advanced Research | 作者: X Luo, T Tian, L Feng, X Yang, L Li, X Tan, W Wu

    IF: 11 | 发表时间: 2023

    Reciprocal inhibition of expression between RAV1 and BES1 modulates plant growth and development in Arabidopsis

    杂志名称: Journal of Integrative Plant Biology | 作者: Yang, Dami, et al.

    IF: 11 | 发表时间: 2023

    Mitogen-activated protein kinase 14-mediated phosphorylation of MaMYB4 negatively regulates banana fruit ripening

    杂志名称: Horticulture Research | 作者: Yang, Yingying, et al.

    IF: 9 | 发表时间: 2023

    Control of grain size in rice by TGW3 phosphorylation of OsIAA10 through potentiation of OsIAA10-OsARF4-mediated auxin signaling

    杂志名称: Cell Reports | 作者: M Ma, SY Shen, C Bai, WQ Wang, XH Feng, JZ Ying

    IF: 9 | 发表时间: 2023

    PeMPK17 interacts with PeMKK7 and participates in para-hydroxybenzoic acid stress resistance by removing reactive oxygen species

    杂志名称: Ecotoxicology and Environmental Safety | 作者: Niu, Yajie, et al.

    IF: 7 | 发表时间: 2023

    Thioredoxin-interacting protein links endoplasmic reticulum stress to inflammatory brain injury and apoptosis after subarachnoid haemorrhage

    杂志名称: J Neuroinflammation | 作者: Zhao Q, Che X, Zhang H, et al

    IF: 6 | 发表时间: 2017

    Apple vacuolar processing enzyme 4 is regulated by cysteine protease inhibitor and modulates fruit disease resistance

    杂志名称: Journal of Experimental Botany | 作者: C Dong, R Li, N Wang, Y Liu, Y Zhang

    IF: 6 | 发表时间: 2022

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