分享产品
重组人成纤维细胞生长因子10,His-标签(无动物源)(FGF-10)
Human FGF-10 Protein, His tag (Animal-Free)
产品特点:
选择规格
买2送1活动进行中!购买任意2个规格产品,免费赠送100μL装一支。
活动截止时间:2024年1月31日
买2送1活动进行中!购买任意2个规格产品,免费赠送100μL装一支。
活动截止时间:2024年1月31日
重组人成纤维细胞生长因子10(FGF-10)蛋白(货号:PRP1913)是一种带有His标签的无动物源重组蛋白,表达于大肠杆菌宿主,专为细胞培养和相关研究设计。该产品以冻干粉形式提供,冻干缓冲液为无菌PBS(pH 7.4),适用于精确调控细胞增殖与分化实验。
FGF-10是一种多功能的间充质上皮信号传导生长因子,主要由子宫内膜基质细胞、成纤维细胞、小管周围细胞、间质细胞和神经胶质细胞分泌。它通过调节胚胎发育、细胞增殖和细胞分化过程,在正常生理和疾病机制中发挥关键作用。FGF-10与癌症和人类遗传疾病密切相关,其信号通路涉及肿瘤发生早期阶段,例如通过影响基质金属蛋白酶(如MMP-7)的活性来介导细胞外基质的分解,从而在肿瘤发展和细胞迁移中扮演重要角色。
该产品主要应用于细胞培养领域,特别适用于研究细胞增殖、分化调控以及发育生物学机制。其在癌症模型(如肿瘤发生研究)、遗传疾病探索和干细胞实验中也具有广泛潜力,支持FGFA和Keratinocyte Growth Factor-2等指标的检测分析。
| 中文名称 | 重组人成纤维细胞生长因子10,His-标签(无动物源)(FGF-10) |
| 英文名称 | Human FGF-10 Protein, His tag (Animal-Free) |
| 产品货号 | PRP1913 |
| 序列 | 氨基酸序列来源于:人成纤维细胞生长因子(Leu40-Ser208)。 |
| 种属 | 人 |
| 活性 | 通过其诱导3T3细胞增殖的能力来衡量。这种效应的 ED₅₀小于8毫微克/毫升。重组人 FGF-10的比活力 > 1.2 x 10⁵I U/mg。 |
| 表达宿主 | 大肠杆菌 |
| 纯度 | >98%,使用SDS-PAGE检测 |
| 产品形式 | 冻干粉,冻干缓冲液为无菌的PBS, pH 7.4。 |
| 分子量 | 19 kDa |
| 蛋白质ID | O15520 |
| 分子 | FGFA, Keratinocyte Growth Factor-2 |
| 检测指标 | FGFA, Keratinocyte Growth Factor-2 |
| 注意事项 | 开盖使用前,请先离心。本产品为冻干粉,推荐用该产品配套的Reconstitution Buffer进行复溶,且浓度不低于100 µg/mL。客户可根据后续的实验需求,进行进一步的稀释。 |
| 保存建议 | 冻干蛋白应在 -20°C 下保存 1 年。复溶后,在 2°C 至 8°C 下最多可保存 1 周。 在含有载体蛋白或稳定剂的缓冲液(如 0.1% BSA、10%FBS、5%HSA )中进一步稀释后,分装的蛋白质应在 -20°C 或 -80°C 下保存 3-6 个月。避免反复冻融循环。 |
| 运输条件 | 冰袋运输(蓝冰) |
| 警告 | 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。 |
Amino acid sequence derived from Human FGF-10 (Leu40-Ser208) (O15520) was expressed with 6×His tag at the C-terminus.
The recombinant Human FGF-10 consists of 169 amino acids and predicts a molecular mass of 20 kDa.
Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 8 ng/mL.
<0.1 EU per 1 μg of the protein by the LAL method.
Lyophilized from PBS, pH 7.4.
Amino acid sequence derived from Human FGF-10 (Leu40-Ser208) (O15520) was expressed with 6×His tag at the C-terminus.
The recombinant Human FGF-10 consists of 169 amino acids and predicts a molecular mass of 20 kDa.
Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 8 ng/mL.
<0.1 EU per 1 μg of the protein by the LAL method.
Lyophilized from PBS, pH 7.4.
Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA, 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA, 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.
Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
暂无相关常见问题。如有疑问,请联系我们的技术支持团队。
请等待最新文献信息更新。
我们的技术团队为您提供全方位的产品支持服务
根据您的研究目标,提供个性化的实验设计方案
经验丰富的技术专家协助您解决实验中遇到的问题
7x24小时在线技术支持,随时为您答疑解惑
提供免费样品试用,让您先试后买更放心