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重组人成纤维生长因子19,His-标签(FGF-19)

Human FGF-19 Protein, His tag

产品货号
PRP1235

产品特点:

  • 超高纯度:经SDS-PAGE验证纯度 > 98%
  • 极低内毒素:LAL法检测每微克蛋白内毒素含量 < 0.1 EU
  • 严格质控:冻干缓冲液为无菌20 mM Tris, 150 mM NaCl (pH8.0)
  • 稳定储存:冻干粉形式支持-20℃以下长期保存
  • 人源特异性:序列来源于人FGF-19基因(UniProt: O95750)
  • 选择规格

    5 μg
    ¥588
    现货(次日发货)
    20 μg
    ¥1498
    现货(次日发货)
    100 μg
    ¥3589
    现货(次日发货)
    1 mg
    ¥请咨询
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    活动截止时间:2024年1月31日

    产品概述

    重组人成纤维生长因子19(His标签)(Human FGF-19 Protein, His tag)是一种由大肠杆菌表达系统生产的重组蛋白,经SDS-PAGE检测纯度大于98%,每微克蛋白内毒素含量低于0.1 EU(LAL法测定),适用于细胞培养及相关机制研究的高纯度细胞因子。

    成纤维细胞生长因子19(FGF-19)是成纤维细胞生长因子家族的分泌型成员,在胎儿大脑、软骨、视网膜及成人胆囊中特异性表达。FGF-19通过与高亲和力受体FGFR1-4结合并激活其酪氨酸激酶活性,参与调节葡萄糖和脂质稳态。作为回肠响应胆汁酸吸收产生的激素,FGF-19在能量消耗调控中发挥核心作用:研究表明其可抵抗饮食诱导的肥胖和胰岛素脱敏,显著改善糖尿病模型动物的胰岛素敏感性及糖脂代谢谱。本产品为重组人源FGF-19蛋白片段(Arg22-Lys216),由195个氨基酸构成,预测分子量21.8 kDa,C端融合His标签便于纯化与检测。

    应用领域

    本品适用于:1)细胞培养体系中代谢调控机制研究;2)FGFR受体信号通路探索;3)肥胖症及糖尿病等代谢性疾病的体外模型构建;4)能量稳态相关药物筛选平台开发。

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    重组人成纤维生长因子19,His-标签(FGF-19)

    产品参数

    中文名称 重组人成纤维生长因子19,His-标签(FGF-19)
    英文名称 Human FGF-19 Protein, His tag
    产品货号 PRP1235
    序列 氨基酸序列来源于:人源 FGF19 (Arg22-Lys216) (O95750) 表达的蛋白片段。
    标签 His-标签
    种属
    活性 Testing in Progress
    蛋白长度 重组人FGF19 由195个氨基酸组成,预测分子量为21.8 KD。
    表达宿主 大肠杆菌
    纯度 > 98 % ,使用SDS-PAGE检测
    产品形式 冻干粉,冻干缓冲液为无菌的20 mM Tris, 150 mM NaCl, pH8.0.
    分子量 21.8 kDa
    基因ID 2255
    蛋白质ID O95750
    蛋白序列链接 FGF19 - Fibroblast growth factor 19 - Homo sapiens (Human) | UniProtKB | UniProt
    分子 FGF-19
    检测指标 FGF-19
    注意事项 开盖使用前,请先离心。本产品为冻干粉,推荐用该产品配套的Reconstitution Buffer进行复溶,且浓度不低于100 µg/mL。客户可根据后续的实验需求,进行进一步的稀释。
    保存建议 本产品为冻干粉形式,建议:将冻干粉产品保存于-20℃以下的干燥环境中。本产品溶解之后,可在4℃保存2-7天,长期使用请置于-20℃以下。为长期保存,推荐添加载体蛋白(0.1% HSA或者BSA),并酌情分装保存,避免反复冻融。
    运输条件 冰袋运输(蓝冰)
    警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

    使用说明

    Sequence

    Amino acid sequence derived from Human FGF-19 (Arg22-Lys216) (O95750) was expressed with a 6×His tag at the N-terminus.

    Protein length

    The recombinant Human FGF-19 consists of 195 amino acids and predicts a molecular mass of 21.8 kDa.

    Protein length

    The recombinant Human FGF-19 consists of 195 amino acids and predicts a molecular mass of 21.8 kDa.

    Biological Activity

    Testing in progress.

    Biological Activity

    Testing in progress.

    Endotoxin

    < 0.1 EU per μg of the protein as determined by the LAL method.

    Endotoxin

    < 0.1 EU per μg of the protein as determined by the LAL method.

    Formulation

    Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0.

    Formulation

    Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0.

    Storage Instructions

    Lyophilized protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

    Storage Instructions

    Lyophilized protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

    Fig. SDS-PAGE analysis of Human FGF-19 Protein.

    Fig. SDS-PAGE analysis of Human FGF-19 Protein.

    Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

    Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

    Usage notes

    Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

    Usage notes

    Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

    Version 20240430
    Abbkine, Inc • USA • www.abbkine.com
    Not for further distribution without written consent. Copyright © Abbkine, Inc.

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