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重组人半乳糖凝集素2蛋白（Human Galectin-2 Protein, His tag, Animal-Free）是一种由大肠杆菌表达、带有His标签的无动物源重组蛋白，采用冻干粉形式，缓冲液为无菌PBS（pH 7.4）。该产品专为细胞培养及细胞因子相关研究设计，通过高灵敏度方法检测其生物活性，适用于细胞类样本的实验应用。
Galectin-2（Gal-2）是凝集素家族成员，结构上与半乳糖凝集素1密切相关，含有一个负责β-半乳糖苷结合作用的碳水化合物识别域（CRD），并以同源二聚体形式发挥生物活性。它在免疫调控中作为促凋亡效应因子，通过caspase-3信号通路作用于活化T细胞，并驱动巨噬细胞向CD40阳性M1极化，从而影响动脉生成抑制和侧支动脉重塑。基于此背景，重组人Galectin-2蛋白在细胞生物学研究中可作为关键工具，用于探索免疫反应、细胞凋亡机制及血管相关病理过程。
本产品主要应用于细胞培养领域，特别适用于免疫细胞研究（如T细胞凋亡机制）、巨噬细胞极化分析、血管生成相关实验，以及细胞因子在心血管疾病和免疫调控中的基础研究。
| 中文名称 | 重组人半乳糖凝集素2,His-标签(无动物源)(GAL2) |
| 英文名称 | Human Galectin-2 Protein, His tag (Animal-Free) |
| 产品货号 | PRP1215 |
| 序列 | 氨基酸序列来源于：人源Galectin-2 Protein (Thr1-Glu132)(P05162) |
| 标签 | His |
| 种属 | 人 |
| 活性 | Measured by its ability to agglutinate human red blood cells. The ED₅₀ for this effect is <20 μg/mL. |
| 蛋白长度 | 重组人Galectin-2 蛋白由132个氨基酸组成，预测分子量为15.5 KD。 |
| 表达宿主 | 大肠杆菌 |
| 纯度 | > 98 % ，使用SDS-PAGE检测 |
| 产品形式 | 冻干粉，冻干缓冲液为无菌的PBS，pH 7.4。 |
| 分子量 | 15.5 kDa |
| 基因ID | 3957 |
| 蛋白质ID | P05162 |
| 蛋白序列链接 | https://www.uniprot.org/uniprotkb/P05162/entry#similar_proteins |
| 分子 | Galectin-2 |
| 检测指标 | Galectin-2 |
| 注意事项 | 开盖使用前，请先离心。本产品为冻干粉，推荐用该产品配套的Reconstitution Buffer进行复溶，且浓度不低于100 µg/mL。客户可根据后续的实验需求，进行进一步的稀释。 |
| 保存建议 | 冻干蛋白应在 -20°C 下保存 1 年。复溶后，在 2°C 至 8°C 下最多可保存 1 周。 在含有载体蛋白或稳定剂的缓冲液（如 0.1% BSA、10%FBS、5%HSA）中进一步稀释后，分装的蛋白质应在 -20°C 或 -80°C 下保存 3-15 个月。避免反复冻融循环。 |
| 运输条件 | 冰袋运输（蓝冰） |
| 警告 | 本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。 |
Amino acid sequence derived from Human Galectin-2 Protein (Thr1-Glu132)(P05162) was expressed with 6×His tag at the N-terminus.
The recombinant Human Galectin-2 Protein consists of 132 amino acids and predicts a molecular mass of 15.5 kDa.
The recombinant Human Galectin-2 Protein consists of 132 amino acids and predicts a molecular mass of 15.5 kDa.
Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is <20 μg/mL.
Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is <20 μg/mL.
<0.1 EU per 1 μg of the protein by the LAL method.
<0.1 EU per 1 μg of the protein by the LAL method.
Lyophilized from PBS, pH 7.4.
Lyophilized from PBS, pH 7.4.
Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA, 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA, 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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