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重组小鼠CXC趋化因子配体9,His-标签(无动物源)(CXCL9)

Mouse CXCL9 Protein, His tag (Animal-Free)

产品货号
PRP1120

产品特点:

  • 高纯度:> 98%,经SDS-PAGE严格检测
  • 低内毒素:<0.1 EU/μg,通过LAL法精确测定
  • 高生物活性:ED50 <0.3 μg/mL,有效趋化BaF3细胞
  • 无动物源成分:最大限度减少外源污染风险
  • 优化储存形式:冻干粉便于运输和长期保存
  • 选择规格

    5 μg
    ¥588
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    20 μg
    ¥1498
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    100 μg
    ¥2969
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    1 mg
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    活动截止时间:2024年1月31日

    产品概述

    PRP1120 重组小鼠CXC趋化因子配体9,His-标签(无动物源)是一种重组蛋白产品,由大肠杆菌表达系统生产,采用His标签纯化工艺,专为细胞类研究设计,特别适用于细胞培养实验,提供高纯度且无动物源的CXCL9蛋白冻干粉。

    C-X-C motif chemokine 9 (CXCL9),也称为γ干扰素诱导的单核因子(MIG),是一种关键的趋化因子,属于CXC家族成员。CXCL9通过结合CXCR3受体,调控免疫细胞的迁移和激活,包括淋巴细胞和巨噬细胞,在炎症反应中发挥核心趋化作用。该分子参与肿瘤增殖与转移过程,是研究免疫调控和疾病机制的理想靶点。PRP1120产品提供高活性重组小鼠CXCL9蛋白,确保在细胞迁移实验中实现可靠、可重复的结果。

    本产品广泛应用于细胞培养、免疫调控研究、炎症模型构建、肿瘤生物学实验及趋化因子信号通路分析等领域。

    重组小鼠CXC趋化因子配体9,His-标签(无动物源)(CXCL9)

    产品参数

    中文名称 重组小鼠CXC趋化因子配体9,His-标签(无动物源)(CXCL9)
    英文名称 Mouse CXCL9 Protein, His tag (Animal-Free)
    产品货号 PRP1120
    序列 氨基酸序列来源于:小鼠源CXCL9 (Thr22-Thr126) (AAA39706.1)
    标签 His
    种属 小鼠
    活性 Measure by its ability to chemoattract BaF3 cells transfected with mouse CXCR3. The ED₅₀ for this effect is <0.3 μg/mL.
    蛋白长度 重组人CXCL9 蛋白由105个氨基酸组成,预测分子量为13.0 KD。
    表达宿主 大肠杆菌
    纯度 > 98 % ,使用SDS-PAGE检测
    产品形式 冻干粉,冻干缓冲液为无菌的PBS,pH 7.4。
    分子量 13.00 kDa
    基因ID 17329
    蛋白质ID AAA39706.1
    蛋白序列链接 https://www.uniprot.org/uniprotkb/P18340/entry#similar_proteins
    分子 CXCL9
    检测指标 CXCL9
    注意事项 开盖使用前,请先离心。本产品为冻干粉,推荐用该产品配套的Reconstitution Buffer进行复溶,且浓度不低于100 µg/mL。客户可根据后续的实验需求,进行进一步的稀释。
    保存建议 冻干蛋白应在 -20°C 下保存 1 年。复溶后,在 2°C 至 8°C 下最多可保存 1 周。 在含有载体蛋白或稳定剂的缓冲液(如 0.1% BSA、10%FBS、5%HSA)中进一步稀释后,分装的蛋白质应在 -20°C 或 -80°C 下保存 3-15 个月。避免反复冻融循环。
    运输条件 冰袋运输(蓝冰)
    警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

    使用说明

    Sequence

    Amino acid sequence derived from Mouse CXCL9 (Thr22-Thr126) (AAA39706.1) was expressed with 6×His tag at the N-terminus.

    Protein length

    The recombinant Mouse CXCL9 consists of 105 amino acids and predicts a molecular mass of 13.0 kDa.

    Biological Activity

    Measure by its ability to chemoattract BaF3 cells transfected with mouse CXCR3. The ED50 for this effect is < 0.3 μg/mL.

    Endotoxin

    <0.1 EU per 1 μg of the protein by the LAL method.

    Formulation

    Lyophilized from PBS, pH 7.4.

    Biological Activity

    Measure by its ability to chemoattract BaF3 cells transfected with mouse CXCR3. The ED50 for this effect is < 0.3 μg/mL.

    Endotoxin

    <0.1 EU per 1 μg of the protein by the LAL method.

    Formulation

    Lyophilized from PBS, pH 7.4.

    Storage Instructions

    Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA, 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.

    Storage Instructions

    Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA, 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.

    Usage notes

    Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.

    Usage notes

    Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.

    Fig. SDS-PAGE analysis of Mouse CXCL9 Protein.

    Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

    Fig. SDS-PAGE analysis of Mouse CXCL9 Protein.

    Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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