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重组人白介素-2,His-标签(无动物源)(IL-2)
Human IL-2 Protein, His tag (Animal-Free)
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活动截止时间:2024年1月31日
买2送1活动进行中!购买任意2个规格产品,免费赠送100μL装一支。
活动截止时间:2024年1月31日
重组人白介素-2蛋白(Human IL-2 Protein, His tag (Animal-Free),货号PRP1086)是一种由大肠杆菌表达系统生产的高纯度重组蛋白,带有C末端6×His标签,无动物源成分,适用于细胞培养及相关研究中IL-2的检测和功能分析。
白细胞介素-2(IL-2)是一种15.5 kDa的免疫调节细胞因子,主要由CD4+ T细胞、CD8+ T细胞、自然杀伤(NK)细胞和树突状细胞表达,在调控NK细胞增殖、细胞毒性、B细胞分化以及T细胞(如幼稚CD4+ T细胞向Th1/Th2分化)中发挥核心作用。重组人IL-2蛋白提供了一种标准化的检测工具,其原理基于IL-2通过结合受体激活细胞信号通路,诱导CTLL-2细胞增殖;该活性的ED₅₀值小于3 ng/mL,比活力大于5 × 10⁶ IU/mg,适用于细胞类样本的定量分析。
本产品主要应用于细胞培养实验、免疫细胞功能研究(如T细胞分化、NK细胞活化分析)、以及生物医药领域的IL-2相关机制探索和药物开发。
| 中文名称 | 重组人白介素-2,His-标签(无动物源)(IL-2) |
| 英文名称 | Human IL-2 Protein, His tag (Animal-Free) |
| 产品货号 | PRP1086 |
| 序列 | 氨基酸序列来源于: 人 IL-2(Ala21-Thr153)(P60568)的氨基酸序列,在C末端带有6×His标签。 |
| 种属 | 人 |
| 活性 | 通过其诱导 CTLL-2 细胞增殖的能力来衡量。这种效应的 ED₅₀ 值为 <3 ng/mL。重组人 IL-2 的比活力大于 5 x 10⁶ IU/mg。 |
| 蛋白长度 | 该蛋白质的计算分子量为 16.4 kDa,在还原条件下,蛋白质的迁移率为 13 kDa(SDS-PAGE 分析)。 |
| 表达宿主 | 大肠杆菌 |
| 纯度 | > 98 % ,使用SDS-PAGE检测 |
| 产品形式 | 该蛋白质是由 0.2 µm 过滤溶液冻干而成,其中含有 1X PBS,pH 值为 8.0。如有任何疑问或特殊要求,请与我们联系。 |
| 分子量 | 16.4 kDa |
| 蛋白质ID | P60568 |
| 蛋白序列链接 | https://www.uniprot.org/uniprot/P60568 |
| 分子 | IL-2 |
| 检测指标 | IL-2 |
| 注意事项 | 开盖使用前,请先离心。本产品为冻干粉,推荐用该产品配套的Reconstitution Buffer进行复溶,且浓度不低于100 µg/mL。客户可根据后续的实验需求,进行进一步的稀释。 |
| 保存建议 | 冻干蛋白应在 -20°C 下保存 1 年。重组后,在 2°C 至 8°C 下最多可保存 1 周。 在含有载体蛋白或稳定剂的缓冲液(如 0.1% BSA、10%FBS、5%HSA)中进一步稀释后,蛋白等分应在 -20°C 或 -80°C 下保存 3-6 个月。避免反复冻融循环。 |
| 运输条件 | 冰袋运输(蓝冰) |
| 警告 | 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。 |
Amino acid sequence derived from Human IL-2 (Ala21-Thr153) (P60568) was expressed with 6×His tag at the C-terminus.
The recombinant Human IL-2 Protein consists of 133 amino acids and predicts a molecular mass of 16.4 kDa.
The recombinant Human IL-2 Protein consists of 133 amino acids and predicts a molecular mass of 16.4 kDa.
Measured by its ability to induce proliferation in CTLL-2 cells. The ED50 for this effect is <3 ng/mL.
Measured by its ability to induce proliferation in CTLL-2 cells. The ED50 for this effect is <3 ng/mL.
<0.01 EU per μg of the protein as determined by the LAL method.
<0.01 EU per μg of the protein as determined by the LAL method.
Lyophilized from sterile PBS, pH 8.0.
Lyophilized from sterile PBS, pH 8.0.
Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA, 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA, 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.
Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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