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重组人核因子κ B受体活化因子配体,His-标签(无动物源)(RANKL)

Huamn RANKL protein, His tag (Animal-Free)

产品货号
PRP1056

产品特点:

  • 高纯度:>98%,经SDS-PAGE严格检测,确保产品一致性
  • 低内毒素水平:每1μg蛋白质中内毒素单位(EU)含量小于0.1,采用LAL法验证
  • 无动物源生产:全程避免动物源性成分,减少免疫原性风险
  • 高生物活性:ED50<10 ng/mL,在RAW264.7细胞中高效诱导破骨细胞分化
  • 冻干粉形式:0.2 µm过滤的1X PBS(pH 8.0)溶液冻干而成,便于储存和复溶
  • 选择规格

    5 μg
    ¥588
    现货(次日发货)
    20 μg
    ¥1498
    现货(次日发货)
    100 μg
    ¥3998
    现货(次日发货)
    1 mg
    ¥请咨询
    请咨询
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    活动截止时间:2024年1月31日

    产品概述

    重组人核因子κ B受体活化因子配体(RANKL),His标签(无动物源),是一种基于大肠杆菌表达系统生产的重组蛋白,包含人源RANKL的特定功能片段(Ala142-Asp317),C端融合6×His标签,用于细胞培养和骨代谢相关研究的高纯度细胞因子。

    RANKL(核因子κ B受体活化因子配体)是TNF细胞因子超家族中的II型跨膜蛋白,其细胞外结构域介导与受体RANK的相互作用,在T细胞富集器官如胸腺和淋巴结中高表达,并在骨重塑、免疫调节及疾病失调过程中发挥核心作用。该重组蛋白通过大肠杆菌高效表达,在RAW264.7细胞模型中展现出强效诱导破骨细胞分化的生物活性,ED50值低于10 ng/mL,为研究骨代谢机制和免疫系统功能提供了可靠工具。

    本产品主要应用于细胞类研究,尤其在一级应用细胞培养领域,如骨代谢分析、破骨细胞分化实验、免疫调控机制探索以及相关疾病模型构建,服务于生物医学和药物研发。

    重组人核因子κ B受体活化因子配体,His-标签(无动物源)(RANKL)

    产品参数

    中文名称 重组人核因子κ B受体活化因子配体,His-标签(无动物源)(RANKL)
    英文名称 Huamn RANKL protein, His tag (Animal-Free)
    产品货号 PRP1056
    序列 氨基酸序列来源于人源: RANKL (Ala142-Asp317)(O14788)表达的蛋白片段,C-末端带有 6×His 标记。
    标签 His
    种属
    活性 通过其在 RAW264.7 细胞中诱导破骨细胞分化的能力来衡量。这种效应的ED50<10 ng/mL。
    蛋白长度 重组人RANKL由176个氨基酸组成,预测分子量为19.9 kDa。
    表达宿主 大肠杆菌
    纯度 > 98 % ,使用SDS-PAGE检测
    产品形式 该蛋白质是由 0.2 µm 过滤溶液冻干而成,其中含有 1X PBS,pH 值为 8.0。如有任何疑问或特殊要求,请与我们联系。
    分子量 20.67 kDa
    蛋白质ID O14788
    蛋白序列链接 http://www.uniprot.org/uniprot/O14788
    分子 RANKL
    注意事项 开盖使用前,请先离心。本产品为冻干粉,推荐用该产品配套的Reconstitution Buffer进行复溶,且浓度不低于100 µg/mL。客户可根据后续的实验需求,进行进一步的稀释。
    保存建议 冻干蛋白应在 -20°C 下保存 1 年。重组后,在 2°C 至 8°C 下最多可保存 1 周。 在含有载体蛋白或稳定剂的缓冲液中进一步稀释(如 0.1%BSA、10%FBS、5%HSA)中进一步稀释,蛋白等分应在 -20°C 或 -80°C 下保存 3-6 个月。避免反复冻融循环。
    运输条件 冰袋运输(蓝冰)
    警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

    使用说明

    Sequence

    Amino acid sequence derived from Human RANKL (Ala142-Asp317)(O14788) was expressed with 6×His tag at the C-terminus.

    Protein length

    The recombinant Human RANKL consists of 176 amino acids and predicts a molecular mass of 19.9 kDa.

    Protein length

    The recombinant Human RANKL consists of 176 amino acids and predicts a molecular mass of 19.9 kDa.

    Biological Activity

    Measure by its ability to induce osteoclast differentiation in RAW264.7 cells. The ED50 for this effect is <10 ng/mL.

    Biological Activity

    Measure by its ability to induce osteoclast differentiation in RAW264.7 cells. The ED50 for this effect is <10 ng/mL.

    Endotoxin

    < 0.1 EU per μg of the protein as determined by the LAL method.

    Endotoxin

    < 0.1 EU per μg of the protein as determined by the LAL method.

    Formulation

    The protein was lyophilized from a 0.2 µm filtered solution containing PBS, pH 8.0.

    Formulation

    The protein was lyophilized from a 0.2 µm filtered solution containing PBS, pH 8.0.

    Storage Instructions

    Lyophilized protein protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4 °C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.

    Storage Instructions

    Lyophilized protein protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4 °C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.

    Usage notes

    Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Human RANKL Protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.

    Usage notes

    Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Human RANKL Protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.

    Fig. SDS-PAGE analysis of Human RANKL protein.

    Fig. SDS-PAGE analysis of Human RANKL protein.

    Note

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

    Note

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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