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山羊抗小鼠IgG二抗,远红外DyLight 800荧光标记

Dylight 800, Goat Anti-Mouse IgG

产品货号
A23910

产品特点:

  • 远红外DyLight 800染料:激发/发射峰~777 nm,光稳定性强,抗淬灭,适用于长时间成像
  • 多场景兼容:支持IF、ICC、FCM、WB等主流蛋白检测技术,适配远红外通道
  • 高纯度亲和纯化:经小鼠IgG(H+L)固相吸附,纯度≥95%,降低非特异性结合
  • 多克隆广谱识别:与小鼠IgG全分子、重链及轻链反应,覆盖所有亚型
  • 即用型液体配方:含PBS缓冲液、BSA稳定剂及50%甘油,-20°C保存1年
  • 选择规格

    100 μL
    ¥298
    现货(当天发货)
    500 μL
    ¥1139
    现货(当天发货)
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    买2送1活动进行中!购买任意2个规格产品,免费赠送100μL装一支。

    活动截止时间:2024年1月31日

    产品概述

    山羊抗小鼠IgG二抗,远红外DyLight 800荧光标记(Dylight 800, Goat Anti-Mouse IgG)是一款专为免疫荧光(IF)、免疫细胞化学(ICC)、流式细胞术(FCM)及Western Blot(WB)等多场景设计的高灵敏度远红外二抗。该抗体以亲和纯化山羊多克隆IgG为骨架,通过共价偶联新一代DyLight 800荧光染料,实现对小鼠来源IgG全分子(重链+轻链)的精准识别,为蛋白类样本的远红外通道检测提供可靠工具。

    在蛋白类研究中,远红外波段(~777 nm)检测可显著降低组织自发荧光与光散射背景,尤其适用于厚组织切片、活体成像及多重标记实验。Abbkine DyLight 800标记的二抗通过固相偶联的小鼠IgG(H+L)轻链进行亲和纯化,蛋白纯度≥95%(SDS-PAGE验证),确保与非免疫球蛋白血清蛋白无交叉反应,同时兼容小鼠IgG各亚型及轻链结构。其工作原理基于抗原-抗体特异性结合,DyLight 800染料在远红外区产生高强度荧光信号,经激光激发后由成像系统捕获,实现低背景、高分辨率的蛋白定位与定量。

    应用领域:适用于细胞/组织免疫荧光成像流式细胞术表型分析Western Blot远红外通道检测多重标记共定位实验,尤其适合需远红外波段检测的蛋白类样本研究。

    山羊抗小鼠IgG二抗,远红外DyLight 800荧光标记

    产品参数

    中文名称 山羊抗小鼠IgG二抗,远红外DyLight 800荧光标记
    英文名称 Dylight 800, Goat Anti-Mouse IgG
    产品货号 A23910
    免疫原 小鼠IgG
    宿主 山羊
    反应性 小鼠
    种属 山羊
    检测类型 IF#ICC#FCM#WB
    克隆类型 多克隆
    亚型 山羊免疫球蛋白IgG
    纯化工艺 亲和纯化:使用固相偶联的小鼠免疫球蛋白IgG(H+L)轻链,蛋白纯度高达>95%(SDS-PAGE检测)。
    偶联物 Dylight 800
    产品形式 液体形式
    储存缓冲液 PBS缓冲液(pH 7.4),含有0.02%叠氮化钠(防腐剂,1%BSA(作为蛋白稳定剂)和50%甘油。
    保存建议 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
    运输条件 冰袋运输(蓝冰)
    其它 该抗体与小鼠IgG全分子反应。它也与小鼠IgG的重链,以及小鼠其它免疫球蛋白的轻链发生反应。它对非免疫球蛋白的血清蛋白没有反应性。但它可能与其它物种的免疫球蛋白有潜在的交叉反应。
    警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

    使用说明

    Application Notes

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:1000 dilutions for most fluorescent applications.

    Storage Buffer

    Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol.

    Storage Buffer

    Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol.

    Storage Instructions

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

    Storage Instructions

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

    Figure 1

    DyLight fluorescent dyes are a new family of dyes with improved brightness. DyLight 488-antibody conjugates are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates. DyLight 549-antibody conjugates shows brighter influoresence than TRITC conjugates. Also, DyLight 594-antibody conjugates are noticeably brighter than Alexa 594 conjugates, and much brighter and more water soluble than Texas Red conjugates.

    Figure 1

    DyLight fluorescent dyes are a new family of dyes with improved brightness. DyLight 488-antibody conjugates are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates. DyLight 549-antibody conjugates shows brighter influoresence than TRITC conjugates. Also, DyLight 594-antibody conjugates are noticeably brighter than Alexa 594 conjugates, and much brighter and more water soluble than Texas Red conjugates.

    Figure 2

    DyLight fluorescent dyes show improved photostability as well. DyLight 488 conjugates fade less than FITC and Cy2 conjugates in mounting media indicating that the DyLight 488 molecule is inherently more photostable in epifluorescence microscopes. DyLight 549 conjugates are about as photostable as Alexa 555 conjugates and slightly more photostable than Cy3 conjugates.

    Figure 2

    DyLight fluorescent dyes show improved photostability as well. DyLight 488 conjugates fade less than FITC and Cy2 conjugates in mounting media indicating that the DyLight 488 molecule is inherently more photostable in epifluorescence microscopes. DyLight 549 conjugates are about as photostable as Alexa 555 conjugates and slightly more photostable than Cy3 conjugates.

    Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

    Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

    常见问题

    Q: 普通荧光二抗能不能做WB呢?应该如何成像呢?

    A: 荧光western blotting的结果需要特殊的仪器检测,即一种荧光成像仪器。即运用荧光成像仪可以对荧光WB的实验结果进行成像和数据采集分析。如果希望应用荧光成像做WB,建议选用Abbkine DyLight 680和DyLight 800荧光二抗,货号分别为A23710、A23720、A23910、A23920。

    Q: Abbkine品牌的二抗的浓度为多少呢?

    A: 0.5 mg/ml。

    Q: 抗体中甘油的作用是什么?

    A: 甘油是可以减慢抗体分子之间的碰撞,起到抗体保护剂的作用。

    文献引用

    TrkA promotes MDM2-mediated AGPS ubiquitination and degradation to trigger prostate cancer progression

    杂志名称: Journal of Experimental & Clinical Cancer Research | 作者: Zhang, Yu, et al

    IF: 13 | 发表时间: 2024

    Arginine methylation of METTL14 promotes RNA N6-methyladenosine modification and endoderm differentiation of mouse embryonic stem cells

    杂志名称: Nat Commun | 作者: Xiaona Liu, Hailong Wang, Xueya Zhao

    IF: 12 | 发表时间: 2021

    NASP Promotes Triple-negative Breast Cancer Progression and Metastasis by Stabilizing YAP in a USP15-Dependent Way.

    杂志名称: International Journal of Biological Sciences | 作者: Zheng, Wenfang, et al.

    IF: 10.000 | 发表时间: 2025

    Platelet Membrane and miR-181a-5p Doubly Optimized Nanovesicles Enhance Cardiac Repair Post-Myocardial Infarction through Macrophage Polarization.

    杂志名称: ACS Applied Materials & Interfaces | 作者: Liu, Dongyue, et al.

    IF: 8.200 | 发表时间: 2025

    Exosomes derived from stem cells from apical papilla promote craniofacial soft tissue regeneration by enhancing Cdc42-mediated vascularization

    杂志名称: Stem Cell Research & Therapy | 作者: Y Liu, X Zhuang, S Yu

    IF: 7 | 发表时间: 2021

    Characterization and chemoproteomic profiling of protein O-GlcNAcylation in SOD1-G93A mouse model.

    杂志名称: Molecular Medicine | 作者: Hao, Yi, et al.

    IF: 6.400 | 发表时间: 2025

    Bacillus thuringiensis cry toxin triggers autophagy activity that may enhance cell death

    杂志名称: Pesticide Biochemistry and Physiology | 作者: Y Yang, X Huang, W Yuan

    IF: 5 | 发表时间: 2021

    CD44 occurring alternative splicing promotes cisplatin resistance and evokes tumor immune response in oral squamous cell carcinoma cells

    杂志名称: Translational Oncology | 作者: X Qiao, L Zhu, R Song, C Shang, Y Guo

    IF: 5 | 发表时间: 2023

    The Pharmacological Effect and Mechanism of Lanthanum Hydroxide on Vascular Calcification Caused by Chronic Renal Failure Hyperphosphatemia

    杂志名称: Front Cell Dev Biol | 作者: Lulu Zhao, Shengnan Wang, Hong Liu

    IF: 5 | 发表时间: 2021

    The Oncopromoting Gene RBM6 Inhibits Prostate Tumour Cell Migration During Epithelial‐to‐Mesenchymal Transition.

    杂志名称: Journal of Cellular and Molecular Medicine | 作者: Liu, Ruoyang, et al.

    IF: 4.200 | 发表时间: 2025

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