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ABL1080 α-Tubulin单克隆抗体（3G5）是一款针对微管骨架核心蛋白α-Tubulin的高特异性小鼠单克隆抗体，采用重组蛋白免疫原制备，经亲和层析纯化后呈即用型液体，广泛适用于Western blot (WB)、免疫组化（IHC-P）、免疫荧光（IF）及免疫沉淀（IP）等多种检测场景，是蛋白表达标准化与定位研究的可靠内参工具。
α-Tubulin与β-Tubulin共同构成真核细胞微管的主要结构单元，微管在细胞形态维持、胞内运输、有丝分裂纺锤体组装等生命活动中发挥关键作用。α-Tubulin由TUBA1A基因编码，在神经系统分化细胞中高表达，其突变可导致神经元迁移缺陷相关的无脑回畸形3型（LIS3）。由于α-Tubulin在物种间高度保守且表达丰度稳定，常被用作全细胞或全组织蛋白表达的内参对照。本抗体（克隆号3G5）识别位于α-Tubulin保守区域的表位，可在人、小鼠、大鼠样本中检测到约52 kDa的清晰条带。
作为经典内参抗体，ABL1080广泛应用于：
| 中文名称 | α-Tubulin单克隆抗体(3G5) |
| 英文名称 | Anti-α-Tubulin Monoclonal Antibody (3G5) |
| 产品货号 | ABL1080 |
| 免疫原 | 重组蛋白 |
| 宿主 | 小鼠 |
| 反应性 | 人#小鼠#大鼠 |
| 检测类型 | WB#IHC#IF#IP |
| 克隆类型 | 单克隆 |
| 亚型 | 小鼠IgG |
| 纯化工艺 | 通过使用特异性免疫原的亲和层析，从小鼠腹水中纯化抗体。 |
| 产品形式 | 液体形式 |
| 基因ID | 7846/10376 |
| 蛋白质ID | Q71U36/P68363 |
| 目标条带分子量 | 52 |
| 蛋白序列链接 | http://www.uniprot.org/uniprot/Q71U36 |
| 分子 | α-Tubulin |
| 储存缓冲液 | PBS缓冲液（pH 7.4），0.02％叠氮化钠（防腐剂）和50％甘油。 |
| 保存建议 | 自发货之日起，-20°C可稳定保存1年。为最大限度的避免损失，请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。 |
| 运输条件 | 冰袋运输（蓝冰） |
| 警告 | 本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。 |
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000-1:10000), IHC (1:50-1:300), IF (1:200), IP (1:200).
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000-1:10000), IHC (1:50-1:300), IF (1:200), IP (1:200).
Storage Buffer: Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage Instructions: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Storage Buffer: Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage Buffer:Storage Instructions: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Storage Instructions:Fig.1. Immunofluorescence analysis of α-Tubulin in Hela with DAPI at 1:100 dilution.
Fig.2. Western blot analysis of α-tubulin express at Hela cell lysate (lane A), Rat brian tissue (lane B), Mouse brain tissue (lane C) with Anti-α-tubulin Monoclonal Antibody (3G5) at dilution 1:5000.
Fig.1. Immunofluorescence analysis of α-Tubulin in Hela with DAPI at 1:100 dilution.
Fig.2. Western blot analysis of α-tubulin express at Hela cell lysate (lane A), Rat brian tissue (lane B), Mouse brain tissue (lane C) with Anti-α-tubulin Monoclonal Antibody (3G5) at dilution 1:5000.
Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Note:Version 201805
Abbkine, Inc • USA • www.abbkine.com
Not for further distribution without written consent. Copyright © Abbkine, Inc.
Version 201805
Abbkine, Inc • USA • www.abbkine.com
Not for further distribution without written consent. Copyright © Abbkine, Inc.
A: 荧光western blotting的结果需要特殊的仪器检测，即一种荧光成像仪器。即运用荧光成像仪可以对荧光WB的实验结果进行成像和数据采集分析。如果希望应用荧光成像做WB，建议选用Abbkine DyLight 680和DyLight 800荧光二抗，货号分别为A23710、A23720、A23910、A23920。
A: 0.5 mg/ml。
A: 甘油是可以减慢抗体分子之间的碰撞，起到抗体保护剂的作用。
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