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山羊抗小鼠IgG二抗，红色DyLight 649荧光标记 (Dylight 649, Goat Anti-Mouse IgG) 是一款专为免疫荧光(IF)、免疫细胞化学(ICC)及流式细胞术(FCM)设计的红色荧光二抗。该抗体由山羊免疫小鼠IgG后制备，经亲和纯化获得高纯度多克隆IgG，并与新一代DyLight 649荧光染料共价偶联，可在远红光区（Ex/Em ≈ 654/673 nm）发出明亮而稳定的红色荧光，为小鼠来源一抗提供高灵敏度的信号放大。
背景知识：在免疫荧光、流式细胞术等蛋白检测体系中，标记二抗是连接一抗与检测信号的关键桥梁。Abbkine系列二抗涵盖酶、生物素及多种荧光染料标记，可兼容WB、IHC、IF、FCM、ELISA等多种应用。本款山羊抗小鼠IgG二抗以固相偶联的小鼠IgG(H+L)为配基进行亲和纯化，蛋白纯度>95%（SDS-PAGE验证），确保对小鼠IgG全分子、重链及轻链具有高度特异性；同时对非免疫球蛋白血清蛋白无交叉反应，仅存在与其它物种IgG的潜在交叉结合，背景更低、信噪比更高。
应用领域：广泛适用于免疫荧光(IF)、免疫细胞化学(ICC)、流式细胞术(FCM)等蛋白检测场景，可与小鼠来源一抗配套，实现细胞表面或胞内蛋白、组织切片抗原、细胞分选标记物的高灵敏度、高分辨率检测。
| 中文名称 | 山羊抗小鼠IgG二抗,红色DyLight 649荧光标记 |
| 英文名称 | Dylight 649, Goat Anti-Mouse IgG |
| 产品货号 | A23610 |
| 免疫原 | 小鼠IgG |
| 宿主 | 山羊 |
| 反应性 | 小鼠 |
| 种属 | 山羊 |
| 检测类型 | IF#ICC#FCM |
| 克隆类型 | 多克隆 |
| 亚型 | 山羊免疫球蛋白IgG |
| 纯化工艺 | 亲和纯化：使用固相偶联的小鼠免疫球蛋白IgG(H+L)轻链，蛋白纯度高达>95%（SDS-PAGE检测）。 |
| 偶联物 | Dylight 649 |
| 产品形式 | 液体形式 |
| 储存缓冲液 | PBS缓冲液（pH 7.4），含有0.02％叠氮化钠（防腐剂，1%BSA（作为蛋白稳定剂）和50％甘油。 |
| 保存建议 | 自发货之日起，-20°C可稳定保存1年。为最大限度的避免损失，请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。 |
| 运输条件 | 冰袋运输（蓝冰） |
| 其它 | 该抗体与小鼠IgG全分子反应。它也与小鼠IgG的重链，以及小鼠其它免疫球蛋白的轻链发生反应。它对非免疫球蛋白的血清蛋白没有反应性。但它可能与其它物种的免疫球蛋白有潜在的交叉反应。 |
| 警告 | 本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。 |
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:1000 dilutions for most fluorescent applications. The Ex/Em of this antibody is 652/672, and IF experiments recommend using a confocal microscope of about 650 nm excitation channel, such as: APC channel.
Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol.
Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol.
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
DyLight fluorescent dyes are a new family of dyes with improved brightness. DyLight 488-antibody conjugates are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates. DyLight 549-antibody conjugates shows brighter influoresence than TRITC conjugates. Also, DyLight 594-antibody conjugates are noticeably brighter than Alexa 594 conjugates, and much brighter and more water soluble than Texas Red conjugates.
DyLight fluorescent dyes show improved photostability as well. DyLight 488 conjugates fade less than FITC and Cy2 conjugates in mounting media indicating that the DyLight 488 molecule is inherently more photostable in epifluorescence microscopes. DyLight 549 conjugates are about as photostable as Alexa 555 conjugates and slightly more photostable than Cy3 conjugates.
DyLight fluorescent dyes are a new family of dyes with improved brightness. DyLight 488-antibody conjugates are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates. DyLight 549-antibody conjugates shows brighter influoresence than TRITC conjugates. Also, DyLight 594-antibody conjugates are noticeably brighter than Alexa 594 conjugates, and much brighter and more water soluble than Texas Red conjugates.
DyLight fluorescent dyes are a new family of dyes with improved brightness. DyLight 488-antibody conjugates are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates. DyLight 549-antibody conjugates shows brighter influoresence than TRITC conjugates. Also, DyLight 594-antibody conjugates are noticeably brighter than Alexa 594 conjugates, and much brighter and more water soluble than Texas Red conjugates.
DyLight fluorescent dyes show improved photostability as well. DyLight 488 conjugates fade less than FITC and Cy2 conjugates in mounting media indicating that the DyLight 488 molecule is inherently more photostable in epifluorescence microscopes. DyLight 549 conjugates are about as photostable as Alexa 555 conjugates and slightly more photostable than Cy3 conjugates.
DyLight fluorescent dyes show improved photostability as well. DyLight 488 conjugates fade less than FITC and Cy2 conjugates in mounting media indicating that the DyLight 488 molecule is inherently more photostable in epifluorescence microscopes. DyLight 549 conjugates are about as photostable as Alexa 555 conjugates and slightly more photostable than Cy3 conjugates.
Version 201805
Abbkine, Inc • USA • www.abbkine.com
Copyright © Abbkine, Inc.
A: 荧光western blotting的结果需要特殊的仪器检测，即一种荧光成像仪器。即运用荧光成像仪可以对荧光WB的实验结果进行成像和数据采集分析。如果希望应用荧光成像做WB，建议选用Abbkine DyLight 680和DyLight 800荧光二抗，货号分别为A23710、A23720、A23910、A23920。
A: 0.5 mg/ml。
A: 甘油是可以减慢抗体分子之间的碰撞，起到抗体保护剂的作用。
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