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山羊抗小鼠IgG二抗,近红外DyLight 680荧光标记
Dylight 680, Goat Anti-Mouse IgG
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活动截止时间:2024年1月31日
山羊抗小鼠IgG二抗,近红外DyLight 680荧光标记(Dylight 680, Goat Anti-Mouse IgG)是一款专为免疫荧光(IF)、免疫细胞化学(ICC)、流式细胞术(FCM)及Western Blot(WB)等多场景设计的多克隆二抗。该抗体以亲和纯化的山羊抗小鼠IgG为核心,经近红外DyLight 680荧光染料高效偶联,可在680 nm激发/700 nm发射通道下呈现明亮且稳定的信号,为蛋白定位、定量及相互作用研究提供高灵敏、低背景的检测方案。
在免疫检测体系中,一抗负责特异性识别目标蛋白,而标记二抗则通过结合一抗的Fc段实现信号放大。Abbkine系列二抗涵盖酶、生物素及多种荧光标记,可灵活适配WB、IHC、IF、FCM及ELISA等应用。本款山羊抗小鼠IgG二抗以固相偶联的小鼠IgG(H+L)为配基进行亲和纯化,纯度经SDS-PAGE验证>95%,确保对小鼠IgG全分子、重链及轻链的高效识别,同时避免与非免疫球蛋白血清蛋白的交叉反应,最大限度降低背景干扰。
适用于细胞或组织切片免疫荧光成像、流式细胞术表型分析、免疫细胞化学定位及Western Blot蛋白表达检测,广泛服务于肿瘤研究、神经科学、免疫学及药物开发等方向。
| 中文名称 | 山羊抗小鼠IgG二抗,近红外DyLight 680荧光标记 |
| 英文名称 | Dylight 680, Goat Anti-Mouse IgG |
| 产品货号 | A23710 |
| 免疫原 | 小鼠IgG |
| 宿主 | 山羊 |
| 反应性 | 小鼠 |
| 种属 | 山羊 |
| 检测类型 | IF#ICC#FCM#WB |
| 克隆类型 | 多克隆 |
| 亚型 | 山羊免疫球蛋白IgG |
| 纯化工艺 | 亲和纯化:使用固相偶联的小鼠免疫球蛋白IgG(H+L)轻链,蛋白纯度高达>95%(SDS-PAGE检测)。 |
| 偶联物 | Dylight 680 |
| 产品形式 | 液体形式 |
| 储存缓冲液 | PBS缓冲液(pH 7.4),含有0.02%叠氮化钠(防腐剂,1%BSA(作为蛋白稳定剂)和50%甘油。 |
| 保存建议 | 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。 |
| 运输条件 | 冰袋运输(蓝冰) |
| 其它 | 该抗体与小鼠IgG全分子反应。它也与小鼠IgG的重链,以及小鼠其它免疫球蛋白的轻链发生反应。它对非免疫球蛋白的血清蛋白没有反应性。但它可能与其它物种的免疫球蛋白有潜在的交叉反应。 |
| 警告 | 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。 |
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:1000 dilutions for most fluorescent applications.
Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol.
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Fig.1. DyLight fluorescent dyes are a new family of dyes with improved brightness. DyLight 488-antibody conjugates are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates. DyLight 549-antibody conjugates shows brighter influuence than TRITC conjugates. Also, DyLight 594-antibody conjugates are noticeably brighter than Alexa 594 conjugates, and much brighter and more water soluble than Texas Red conjugates.
Fig.1. DyLight fluorescent dyes are a new family of dyes with improved brightness. DyLight 488-antibody conjugates are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates. DyLight 549-antibody conjugates shows brighter influuence than TRITC conjugates. Also, DyLight 594-antibody conjugates are noticeably brighter than Alexa 594 conjugates, and much brighter and more water soluble than Texas Red conjugates.
Fig.2. DyLight fluorescent dyes show improved photostability as well. DyLight 488 conjugates fade less than FITC and Cy2 conjugates in mounting media indicating that the DyLight 488 molecule is inherently more photostable in epifluorescence microscopes. DyLight 549 conjugates are about as photostable as Alexa 555 conjugates and slightly more photostable than Cy3 conjugates.
Fig.2. DyLight fluorescent dyes show improved photostability as well. DyLight 488 conjugates fade less than FITC and Cy2 conjugates in mounting media indicating that the DyLight 488 molecule is inherently more photostable in epifluorescence microscopes. DyLight 549 conjugates are about as photostable as Alexa 555 conjugates and slightly more photostable than Cy3 conjugates.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
A: 荧光western blotting的结果需要特殊的仪器检测,即一种荧光成像仪器。即运用荧光成像仪可以对荧光WB的实验结果进行成像和数据采集分析。如果希望应用荧光成像做WB,建议选用Abbkine DyLight 680和DyLight 800荧光二抗,货号分别为A23710、A23720、A23910、A23920。
A: 0.5 mg/ml。
A: 甘油是可以减慢抗体分子之间的碰撞,起到抗体保护剂的作用。
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