×

兔抗山羊IgG二抗,橙红色DyLight 594荧光标记

Dylight 594, Rabbit Anti-Goat IgG

产品货号
A23430

产品特点:

  • 采用亲和纯化工艺,蛋白纯度≥95%(SDS-PAGE验证),降低非特异背景
  • 偶联DyLight 594荧光基团,橙红色发射光与常用绿色/远红色染料形成理想多色组合
  • 多克隆抗体设计,可识别山羊IgG全分子及轻链,兼容多种一抗亚型
  • 液体即用型配方,含BSA稳定剂与叠氮化钠防腐,避免反复冻融导致的活性损失
  • 选择规格

    100 μL
    ¥298
    现货(当天发货)
    500 μL
    ¥1139
    现货(当天发货)
    🎉 限时优惠

    买2送1活动进行中!购买任意2个规格产品,免费赠送100μL装一支。

    活动截止时间:2024年1月31日

    产品概述

    兔抗山羊IgG二抗,橙红色DyLight 594荧光标记(Dylight 594, Rabbit Anti-Goat IgG)是一款专为免疫荧光(IF)免疫细胞化学(ICC)流式细胞术(FCM)设计的高性能多克隆二抗。该抗体由兔源IgG制备,经亲和纯化工艺偶联DyLight 594荧光染料,可在550 nm激发、570–590 nm发射范围内呈现明亮橙红色荧光,适用于山羊来源一抗的精准信号放大。

    在免疫荧光实验中,一抗与目标抗原结合后,需借助荧光标记的二抗实现可视化。本抗体以完整山羊IgG分子为免疫原,经兔宿主高免血清制备,可特异性识别山羊IgG的重链及轻链,同时避免与非免疫球蛋白血清蛋白的非特异结合。DyLight 594作为新一代荧光染料,其高量子产率抗淬灭特性显著优于传统染料,确保在长时间激发或高功率激光照射下仍保持信号稳定。

    应用领域

    适用于细胞或组织水平的免疫荧光成像(如共聚焦显微镜、高内涵筛选)、流式细胞术多色分析,以及ICC实验中的抗原定位研究。特别推荐用于山羊源一抗标记的小鼠、大鼠或人源样本检测,需配合封闭血清优化以降低潜在交叉反应。

    兔抗山羊IgG二抗,橙红色DyLight 594荧光标记

    产品参数

    中文名称 兔抗山羊IgG二抗,橙红色DyLight 594荧光标记
    英文名称 Dylight 594, Rabbit Anti-Goat IgG
    产品货号 A23430
    免疫原 山羊IgG
    宿主
    反应性 山羊
    种属
    检测类型 IF#ICC#FCM
    克隆类型 多克隆
    亚型 兔源IgG
    纯化工艺 亲和纯化:使用固相偶联的山羊免疫球蛋白IgG(H+L)轻链,蛋白纯度高达>95%(SDS-PAGE检测)。
    偶联物 Dylight 594
    产品形式 液体形式
    储存缓冲液 PBS缓冲液(pH 7.4),含有0.02%叠氮化钠(防腐剂,1%BSA(作为蛋白稳定剂)和50%甘油。
    保存建议 自发货之日起,-20°C可稳定保存1年。为最大限度的避免损失,请在打开管盖之前融化抗体并离心。我们建议使用前分装以避免反复冻融。
    运输条件 冰袋运输(蓝冰)
    其它 该抗体与山羊IgG全分子反应。它也与山羊IgG的重链,以及山羊其它免疫球蛋白的轻链发生反应。它对非免疫球蛋白的血清蛋白没有反应性。但它可能与其它物种的免疫球蛋白有潜在的交叉反应。
    警告 本文列出的产品仅供研究使用,不适用于人类或临床诊断。我们产品所推荐应用,不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为,我们不承担任何责任。

    使用说明

    Application Notes

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:1000 dilutions for most fluorescent applications.

    Storage Buffer

    Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol.

    Storage Instructions

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

    Fig.1. DyLight fluorescent dyes are a new family of dyes with improved brightness. DyLight 488-antibody conjugates are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates. DyLight 549-antibody conjugates shows brighter influuence than TRITC conjugates. Also, DyLight 594-antibody conjugates are noticeably brighter than Alexa 594 conjugates, and much brighter and more water soluble than Texas Red conjugates.

    Fig.1. DyLight fluorescent dyes are a new family of dyes with improved brightness. DyLight 488-antibody conjugates are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates. DyLight 549-antibody conjugates shows brighter influuence than TRITC conjugates. Also, DyLight 594-antibody conjugates are noticeably brighter than Alexa 594 conjugates, and much brighter and more water soluble than Texas Red conjugates.

    Fig.2. DyLight fluorescent dyes show improved photostability as well. DyLight 488 conjugates fade less than FITC and Cy2 conjugates in mounting media indicating that the DyLight 488 molecule is inherently more photostable in epifluorescence microscopes. DyLight 549 conjugates are about as photostable as Alexa 555 conjugates and slightly more photostable than Cy3 conjugates.

    Fig.2. DyLight fluorescent dyes show improved photostability as well. DyLight 488 conjugates fade less than FITC and Cy2 conjugates in mounting media indicating that the DyLight 488 molecule is inherently more photostable in epifluorescence microscopes. DyLight 549 conjugates are about as photostable as Alexa 555 conjugates and slightly more photostable than Cy3 conjugates.

    Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

    Note: The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

    常见问题

    Q: 该荧光抗体的激发波长和发射波长分别为多少?

    A: 激发波长为593nm,发射波长为618nm。

    Q: 普通荧光二抗能不能做WB呢?应该如何成像呢?

    A: 荧光western blotting的结果需要特殊的仪器检测,即一种荧光成像仪器。即运用荧光成像仪可以对荧光WB的实验结果进行成像和数据采集分析。如果希望应用荧光成像做WB,建议选用Abbkine DyLight 680和DyLight 800荧光二抗,货号分别为A23710、A23720、A23910、A23920。

    Q: Abbkine品牌的二抗的浓度为多少呢?

    A: 0.5 mg/ml。

    Q: 抗体中甘油的作用是什么?

    A: 甘油是可以减慢抗体分子之间的碰撞,起到抗体保护剂的作用。

    文献引用

    Astilbin ameliorates depressive-like behavior caused by postnatal immune activation through Menin-regulated astrocyte inflammation

    杂志名称: J Affect Disord | 作者: Yao Y, Man L, Du J, Wu D, Yang L, Peng F, Han L, Zhao T, Zhou W,

    IF: 5 | 发表时间: 2022

    Genistein promotes M1 macrophage apoptosis and reduces inflammatory response by disrupting miR-21/TIPE2 pathway

    杂志名称: SAUDI PHARMACEUTICAL JOURNAL | 作者: Cong L,  Xie X,  Liu S

    IF: 5 | 发表时间: 2022

    IDO1 as a new immune biomarker for diabetic nephropathy and its correlation with immune cell infiltration

    杂志名称: Int Immunopharmacol | 作者: Kuipeng Yu ,Dengren Li , Fuping Xu

    IF: 4 | 发表时间: 2021

    Exosomes derived from bone marrow mesenchymal stem cells protect the injured spinal cord by inhibiting pericyte pyroptosis

    杂志名称: Neural Regen Res | 作者: Zhou Y, Wen LL, Li YF, Wu KM, Duan RR, Yao YB, Jing LJ, Gong Z, Teng JF, Jia YJ,

    IF: 3 | 发表时间: 2022

    Genistein alleviates chronic vascular inflammatory response via the miR‑21/NF‑κB p65 axis in lipopolysaccharide‑treated mice

    杂志名称: Mol Med Rep | 作者: Xie X, Cong L, Liu S, Xiang L, Fu X,

    IF: 2 | 发表时间: 2021

    相关资讯

    专业技术支持

    我们的技术团队为您提供全方位的产品支持服务

    实验方案设计

    根据您的研究目标,提供个性化的实验设计方案

    问题排查

    经验丰富的技术专家协助您解决实验中遇到的问题

    在线技术咨询

    7x24小时在线技术支持,随时为您答疑解惑

    免费样品申请

    提供免费样品试用,让您先试后买更放心

    400电话
    在线咨询
    技术支持
    公众号
    公众号
    返回顶部